別品名 |
Nano-Trap, Nano Trap, Hemagglutinin tag, HA, HA-tag, HA-tag Trap Magnetic Particles
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免疫動物 |
Alpaca
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構成内容 |
● HA-Trap Agarose: 20 rxns (500 ul slurry) ● Lysis buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5 % Nonidet P40 Substitute, 0.09 % sodium azide): 30 mL ● RIPA buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.1 % SDS,1 % Triton X-100, 1 % Deoxycholate, 0.09 % sodium azide): 30 mL ● Wash buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.05 % Nonidet P40 Substitute, 0.5 mM EDTA, 0.018 % sodium azide): 50 mL (after dilution with 40 mL H2O) ● Dilution buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.018 % sodium azide): 50 mL (after dilution with 40 mL H2O) ● Acidic elution buffer (200 mM glycine pH 2.5): 3x 1 mL
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その他 |
[備考]旧Chromotek(クロモテック、ドイツ)社商品。2020年10月よりProteintech Group(プロテインテック、米国)社ブランドの一部になりました。
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The HA-Trap Agarose Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.
Co-IP using HA-Trap Agarose Kit followed by multiplexed WB of TOM70-HA and HSP90 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with full-length TOM70-HA construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. TOM70 Monoclonal Antibody (66593-1-Ig), Multi-rAB CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM002), HSP90 Polyclonal Antibody (13171-1-AP), and Multi-rAb CoraLite Plus 750-Goat Anti Rabbit Recombinant Secondary Antibody (RGAR006) were used in the WB analysis.
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The HA-Trap Agarose Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.
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メーカー |
品番 |
包装 |
PGI
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ATAK-20
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20 RXN
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希望販売価格 |
¥122,000 ▼ キャンペーン価格 ¥91,500(〜2025/01/31)
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※表示価格について
当社在庫 |
あり
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法規制 |
安・化管
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保存温度 |
4℃禁凍結
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