| 別品名 |
Nano-Trap, Nano Trap, polyubiquitin B, UBB, ubiquitin, ubiquitin B
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| 適用 |
Immunoprecipitation
Co-Immunoprecipitation
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| 免疫動物 |
Alpaca
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| 構成内容 |
● Ubiquitin-Trap Magnetic Agarose, Anti-ubiquitin VHH cross-linked with magnetic agarose beads(品番: utma-20品番: gta-20: Storage buffer: 20 % ethanol)
● Lysis buffer(10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5 % Nonidet P40 Substitute, 0.09 % sodium azide): 30 mL
● RIPA buffer(10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.1 % SDS,1 % Triton X-100, 1 % deoxycholate, 0.09 % sodium azide): 30 mL
● Dilution buffer(10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.018 % sodium azide): 50 mL (after dilution with 40 mL H2O)
● Wash buffer(10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.05 % Nonidet P40 Substitute, 0.5 mM EDTA, 0.018 % sodium azide): 50 mL (after dilution with 40 mL H2O)
● Acidic elution buffer(200 mM glycine pH 2.5): 3x 1 mL
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| その他 |
[備考]旧Chromotek（クロモテック、ドイツ）社商品。2020年10月よりProteintech Group（プロテインテック、米国）社ブランドの一部になりました。
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The ubiquitin-trap magnetic agarose (utma) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from human (HEK293T), mouse (C2C12), and hamster (CHO) cell lines treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.
The ubiquitin-trap magnetic agarose (utma) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from dog (MDCK) cells, spinach (Spinacia oleracea), and baker's yeast (Saccharomyces cerevisiae) treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.
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The ubiquitin-trap magnetic agarose (utma) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from human (HEK293T), mouse (C2C12), and hamster (CHO) cell lines treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.
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| メーカー |
品番 |
包装 |
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PGI
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UTMAK-20
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20 RXN
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 法規制 |
安・化管
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| 保存温度 |
４℃禁凍結
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