Anti keratin 8 chain  

Fig.1 Western blot analysis
Whole cell lysate prepared from DLD-1 cells was immunoblotted with RL273 antibody(1:200 dilution).
The antibody detected a polypeptide band for keratin 8 at approximately 50 kDa.
DLD-1 is a human colorectal adenocarcinoma cell line.

Fig.2 Immunofluorescence microscopy of rabbit colon and DLD-1 cells.
A frozen section of rabbit colon was stained by RL273 antibody at 1:250 dilution (A). The absorptive epithelial cells and mucosal gland cells were decorated by the antibody. Panel B shows the phase contrast image of A. Bar = 100um.
Acetone-fixed DLD-1 cells were stained by RL273 antibody at 1:250 dilution (C), showing the filamentous networks of keratins (green). Cell nuclei were visualized by DAPI (blue). Bar = 25um.