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The human IGF1 recombinant protein (12.5ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: IGF1 transfected 293T cell lysate
ICC/IF analysis of IGF1 in PC3 cells. The cell was stained with ATGA0311 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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The human IGF1 recombinant protein (12.5ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: IGF1 transfected 293T cell lysate
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| 別品名 |
Insulin-like growth factor-1, Insulin-like growth factor-1, IGF-IA, Somatomedin-C, MGF, IBP1, IGF1, IBP1, IGF-1, Insulin-like growth factor-1 IGF IA, IGF IB, IGF1, IGFI, IGFIA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Mechano growth factor, Somatomedin C,
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Mouse
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| クローン |
AT6F8
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| 抗体クラス |
IgG2bκ
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| 抗原部位 |
a.a.49-118
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| Accession No.(Gene/Protein) |
NP_000609, P05019
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| 概要 |
Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. IGF-1 has also been referred to as a Sulfation factor and its effects were termed nonsuppressible insulin-like activity (NSILA) in the 1970s. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin, is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7, 649 daltons.
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| 参考文献 |
Hoppener J.W., et al. (1985) Hum Genet 69(2): 157-160
Jansen M., et al. (1983) Nature 306(5943): 609-611
Salmon W., et al. (1957) J Lab Clin Med 49(6): 825-836
Keating G.M., (2008) BioDrugs 22(3): 177-188
Rinderknecht E., et al. (1978) J Biol Chem 253(8): 2769-2776
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| メーカー |
品番 |
包装 |
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ATG
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ATGA0311
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100 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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