| 別品名 |
HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Flag Western Blot, Western Blotting Kit, Chemiluminescent Flag Kit, Immunoblotting Kit, FLAG(R), HRP Anti FLAG
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| 種由来 |
Mouse
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| 標識物 |
Horseradish Peroxidase
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| 適用 |
Western Blot
Immunoprecipitation
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| 抗体クラス |
IgG
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| 交差種 |
Mouse
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| Tag情報 |
FLAG
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Kong, D., L. Xu, Y. Yu, W. Zhu, D.W. Andrews, Y. Yoon, and T.H. Kuo. 2005. Regulation of Ca2+-induced permeability transition by BCL-2 is antagonized by Drp1 and hFis1. Molecular and Cellular Biochemistry. 272: 187-199. (Rabbit IgG TrueBlot, PubMed) DiPerna, G., J. Stack, A.G. Bowie, A. Boyd, G. Kotwal, Z. Zhang, S. Arvikar, E. Latz, K.A. Fitzgerald, and W.L. Marshall. 2004. Poxvirus protein N1L targets the I-kappaB Kinase complex, inhibits signaling to NF-kappaB by the Tumor Necrosis Factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by Toll-like Receptors. J. Biol. Chem. 279: 36570-36578. (Rabbit IgG TrueBlot, PubMed) Zhang, X., Y. Ozawa, H. Lee, Y. Wen, T. Tan, B. Wadzinski, and E. Seto. 2005. Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4. Genes & Development. 19: 827-839. (Rabbit IgG TrueBlot, PubMed) Lehtonen, S., E. Lehtonen, K. Kudlicka, H. Holthofer, and M.G. Farquhar. 2004. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am J Pathol. 165:923-936. (Rabbit IgG TrueBlot, PubMed) Tyagi A, Agarwal C, Harrison G, Glode LM, Agarwal R. 2004. Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages. Carcinogenesis. 25: 1711-20. (Mouse IgG TrueBlot, PubMed) Antibodies, A Laboratory Manuel. Ed Harlow and David Lane, eds. Cold Spring Harbor Press. 1988. Chapter 12 gives an excellent overview of Western Blotting techniques, including India Ink staining. Current protocols in Molecular Biology. J. Ausebel, et al, eds. John Wiley and Sons, New York. Gives a complete protocol of Western Blotting and Dot Blotting. Molecular Cloning: A Laboratory Manuel. 2nd Edition. J. Sambrook, E.F. Fritsch and T. Maniatis, eds. Cold Spring Harbor Press, 1989. Chapter 18 gives detailed protocols for both the production of cell lysates and electrophoresis and blotting of proteins
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Mouse TrueBlotR IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti Caspase 7 using Mouse TrueBlotR ULTRA: Anti Mouse Ig HRP (Lane 1) or conventional HRP conjugated anti mouse antibody (Lane 2) note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re immunoblotted using conventional HRP conjugated anti mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlotR ULTRA: Anti Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
This product is assembled as a kit. See attached protocol or CofA for further details.
Monoclonal Antibody to detect FLAGTM conjugated proteins detects both C terminal linked and N terminal linked FLAGTM tagged recombinant proteins by western blot.
Immunoprecipitation of VHH-EGFR-FLAG single chain antibody. 500 ug of Hela Whole Cell Lysate (p/n W09-000-364) spiked with 1 ug of VHH-EGFR-FLAG single chain antibody was immunoprecipitated with 1 ug of Mouse anti-FLAGR Monoclonal Antibody (p/n 200-301-383) overnight at 4C. Western Blotting of Immunoprecipitation product followed. Lane 1 - VHH-EGFR-FLAG Immunoprecipitation. Lane 2 - VHH-EGFR-FLAG Pre-Cleared Lysate (prior to immunoprecipitation). Load: 2 uL per lane. Primary Antibody: Mouse anti-FLAGR Monoclonal Antibody at 1:1,000 o/n at 4C. Secondary Antibody: Mouse TrueblotR ULTRA: Anti-Mouse Ig HRP at 1:2,000 for 1 HR at RT. Block: MB-070 for 2 HR at RT. Predicted/Observed size: 11 kDa, 11 kDa for VHH-EGFR-FLAG single chain antibody.
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Mouse TrueBlotR IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti Caspase 7 using Mouse TrueBlotR ULTRA: Anti Mouse Ig HRP (Lane 1) or conventional HRP conjugated anti mouse antibody (Lane 2) note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re immunoblotted using conventional HRP conjugated anti mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlotR ULTRA: Anti Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
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| メーカー |
品番 |
包装 |
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RKL
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88-8887-383
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1 KIT
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃と−２０℃
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