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Fig.1. Identification of CALR protein by western blotting with anti-CALR antibody.
Embryonic fibroblast cells prepared from Calr -/- mouse were transfected with a plasmid expressing Calr. The cell lysate was analyzed by western blotting with anti-CALR antibody at 1/500 dilution.
1. Mock-infected cell lysate as a negative control.
2. Cell lysate transfected with a plasmid expressing Calr.
Fig.2. Immunoprecipitation of CALR protein with anti-CALR antibody.
Lysates of wild-type mouse testis were immunoprecipitated with anti-CALR antibody and the precipitates were analyzed by western blotting with the same antibody.
1. Input testis lysate
2. Precipitated with preimmune serum
3. Precipitated with anti-CALR antibody
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