Anti Histone H3 K79me1  

Immunofluorescence of Histone H3 [monomethyl Lys79-Me1]: Histone H3 K79me1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Western Blot of Histone H3 [monomethyl Lys79] (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 1: HeLa cell lysates. Lane 2: NIH/3T3 cell lysates. Lane 3: Cos 7 cell lysates. Load: 35 μg per lane. Primary antibody: Histone H3 K79-Me1 antibody at 0.5μg/mL for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 K79-Me1.

Dot Blot of Rabbit Histone H3 [monomethyl Lys79] Antibody. Lane 1: K79 unmodified. Lane 2: K79-Me1. Lane 3: K79-Me2. Lane 4: K79-Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 K79-Me1 antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.

Chromatin Immunoprecipitation of Histone H3 [monomethyl Lys79] Antibody: Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of H3 K79Me1 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.