| 別品名 |
AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
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| 種由来 |
Human
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| 標識物 |
Phycoerythrin -R
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Enzyme Linked Immunosorbent Assay
Dot Blot
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| 免疫動物 |
Mouse
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| 抗体クラス |
IgG1κ
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| クローン |
25F6.F6.D8
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| 交差種 |
Human
Mouse
Rat
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| GENE ID |
10000
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| Accession No.(Gene/Protein) |
NP_001193658, Q9Y243
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| Gene Symbol |
AKT3
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| 純度 |
Ig-PA
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| 形状 |
凍結乾燥品
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| 参考文献 |
Madhunapantula SV, et al.The Akt signaling pathway: An emerging therapeutic target in malignant melanoma. Cancer Biol Ther. 2011 Dec 15;12(12)
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry of Mouse Monoclonal anti AKT3 Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: anti AKT3 at 2ug/ml for 1.5 hour @ room Temp. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45 RT.
Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.
Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.
Western Blot of Mouse Anti-AKT3 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 ug per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56 kDa for AKT3.
Western Blot of Mouse anti-AKT3 antibody. Lane 1: Control. Lane 2: Rapa. Lane 3: T50. Lane 4: T250. Lane 5: Control. Lane 6: Rapa. Lane 7: T50. Lane 8: T250. Lane 9: AKT3 null. Load: 35 ug per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56 kDa for AKT3.
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Immunohistochemistry of Mouse Monoclonal anti AKT3 Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: anti AKT3 at 2ug/ml for 1.5 hour @ room Temp. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45 RT.
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| メーカー |
品番 |
包装 |
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RKL
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200-308-E75
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃
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