別品名 |
AKT 1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
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種由来 |
Human
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標識物 |
Biotin
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精製度 |
Ig fraction - Protein A
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免疫動物 |
Mouse
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抗体クラス |
IgG2aκ
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クローン |
14E5.A2.B2.H9
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交差種 |
Human Mouse Rat
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GENE ID |
207
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Accession No.(Gene/Protein) |
NP_001014431, P31749
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Gene Symbol |
AKT1
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純度 |
Ig-PA
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形状 |
凍結乾燥品
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参考文献 |
Madhunapantula SV, et al.The Akt signaling pathway: An emerging therapeutic target in malignant melanoma. Cancer Biol Ther. 2011 Dec 15;12(12)
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Flow Cytometry of Mouse anti AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 ug/mL for 20 min at 4C.
Plate was coated with monoclonal anti AKT1 antibody (capture antibody) followed by incubation with recombinant AKT1 (p/n 009-001-P21), AKT2 (p/n 009-001-P22), AKT3 (p/n 009-001-P23) proteins. Binding was detected with biotinylated monoclonal anti-AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3.
Western Blot of Mouse Anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.
Western Blot of Mouse Anti-AKT1 antibody. Lane 1: AKT-1 Null. Lane 2: WT. Lane 3: MEF #1. Lane 4 : A549 (p/n W09-001-GX4). Lane 5: Calu-1. Lane 6: PC-3 (p/n W09-001-GV6). Lane 7: HepG2 (p/n W09-001-GJ5). Lane 8: Jurkat (p/n W09-001-370). Lane 9: SKOV3 (p/n W09-001-GX9). Lane 10: HEK293T (p/n W09-001-GX5). Lane 11: C2C12 (p/n W10-001-GL7). Load: 20 ug per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 56 kDa for AKT1. Other band(s): none.
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Flow Cytometry of Mouse anti AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 ug/mL for 20 min at 4C.
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メーカー |
品番 |
包装 |
RKL
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200-306-I51
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-70℃
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