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Western Blot of Mouse Anti-AKT1 Fluorescein Conjugated Antibody.Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: AKT1 Recombinant Protein (0.05μg). Lane 3: AKT2 Recombinant Protein (0.05μg). Lane 4: AKT3 Recombinant Protein (0.05μg). Lane 5: HEPG2 Whole Cell Lysate (20μg). Lane 6: C2C12 Whole Cell Lysate (20μg). Lane 7: A549 Whole Cell Lysate (20μg). Primary Antibody: Mouse Anti-AKT1 FITC at 1.0μg/mL overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse HRP (p/n 610-403-C46) at 1:40,000 for 30mins at RT.Blocking: Fluorescent Buffer (p/n MB-073) for 1hr at RT.Predicted MW: ~56kDa. Exposure: 0.5 sec.
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Lane 4: Molecular Weight Marker. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.
Western Blot of Mouse Anti-AKT1 antibody. Lane 1: Molecular Weight Marker. Lane 2: LnCap lysate (p/n W09-001-GJ9). Lane 3: Jurkat lysate (p/n W09-001-370). Lane 4: MDA-MB 468 lysate (p/n W09-001-GG9). Load: 5 μg per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Mouse secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT1.
ELISA of Mouse Monoclonal anti-AKT1 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti-AKT1 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Mouse monoclonal anti-AKT1 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100).
Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 μg/mL for 20 min at 4°C.
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Western Blot of Mouse Anti-AKT1 Fluorescein Conjugated Antibody.Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: AKT1 Recombinant Protein (0.05μg). Lane 3: AKT2 Recombinant Protein (0.05μg). Lane 4: AKT3 Recombinant Protein (0.05μg). Lane 5: HEPG2 Whole Cell Lysate (20μg). Lane 6: C2C12 Whole Cell Lysate (20μg). Lane 7: A549 Whole Cell Lysate (20μg). Primary Antibody: Mouse Anti-AKT1 FITC at 1.0μg/mL overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse HRP (p/n 610-403-C46) at 1:40,000 for 30mins at RT.Blocking: Fluorescent Buffer (p/n MB-073) for 1hr at RT.Predicted MW: ~56kDa. Exposure: 0.5 sec.
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| 別品名 |
mouse anti-AKT1 antibody FITC conjugation, fluorescein conjugated mouse anti-AKT-1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Mouse
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| クローン |
5E5.F5.D7
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| 抗体クラス |
IgG2aκ
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| 標識物 |
Fluorescein Isothiocyanate
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| 精製度 |
Ig fraction - Protein A
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| GENE ID |
207
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| Accession No.(Gene/Protein) |
NP_001014431.1, P31749
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| Gene Symbol |
AKT1
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| 純度 |
Ig-PA
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| 参考文献 |
Madhunapantula SV, et al.The Akt signaling pathway: An emerging therapeutic target in malignant melanoma. Cancer Biol Ther. 2011 Dec 15;12(12)
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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200-302-J34
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-７０℃
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