別品名 |
DL680, TrueBlot, DL680 TrueBlot ULTRA, DyLightTM 680 TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Fluorescent TrueBlot, Rb TrueBlot
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種由来 |
Rabbit
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標識物 |
DyLightTM 680
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精製度 |
Affinity Purified
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適用 |
Western Blot Immunohistochemistry Immuno Fluorescence Flow Cytometry Fluorescence based plate assays
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免疫動物 |
Mouse
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抗体クラス |
IgG
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クローン |
eB182
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交差種 |
Rabbit
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純度 |
Ig-PG
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形状 |
凍結乾燥品
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参考文献 |
[Pub Med ID]29207086, 29436615, 36727462, 34610301, 34741801
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rabbit TrueBlotR IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti human Stat1 and immunoprecipitated using Protein G, Protein A and Anti Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti Stat1 using Rabbit TrueBlotR: Anti Rabbit IgG HRP
Western Blot of Fluorescent TrueBlotR: Anti-Rabbit IgG DyLight 680 Conjugated. Lane 1: Rabbit IgG, Non-denatured. Lane 2: Rabbit IgG, Denatured. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescent TrueBlotR: Anti-Rabbit IgG DyLight 680 Conjugated antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted: 160 kDa for non-denatured; observed: 170-180 kDa for non-denatured. Band migrates at slightly higher molecular weight.
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Rabbit TrueBlotR IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti human Stat1 and immunoprecipitated using Protein G, Protein A and Anti Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti Stat1 using Rabbit TrueBlotR: Anti Rabbit IgG HRP
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メーカー |
品番 |
包装 |
RKL
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18-4416-32
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100 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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法規制 |
毒
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保存温度 |
4℃
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