別品名 |
H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R2me1
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抗原部位 |
N-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Immuno Fluorescence Dot Blot
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免疫動物 |
Rabbit
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交差種 |
Human Mouse Rat
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翻訳後修飾 |
メチル化
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Accession No.(Gene/Protein) |
Q71DI3
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Gene Symbol |
HIST2H3C
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形状 |
滅菌済み液状品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence of Histone H3 K27me3 Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 K27me3 antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 K27me3 is nuclear and chromosomal. Staining: Histone H3 K27me3 is expressed green and the nuclei are counterstained blue with DAPI.
Dot Blot of Histone H3 K27 Me3 Antibody. Lane 1: Non Modified Peptide. Lane 2: Histone H3 K27-Me1 Peptide. Lane 3: Histone H3 K27-Me2 Peptide. Lane 4: Histone H3 K27-Me3 Peptide. Lane 5: Histone H3 K27-Ac Peptide. Dilution: 0.1μg/mL. Blocking: 5% Blotto at RT 30 min. Exposure time: 13 sec.
Western Blot of Histone H3 K27me3 Antibody. Western Blot analysis against untreated cell extracts. Lane 1: Molecular Weight Marker. Lane 2: HeLa cell lysates (p/n W09-000-364). Lane 3: 3T3 cell lysates (p/n W10-000-358). Lane 4: PC12 cell lysates (p/n W12-001-GL9). Lane 5: A431 cell lysate (p/n W09-000-361). Primary antibody: Histone H3 K27me3 antibody at 1.0 ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: (p/n MB-073) BlockOut overnight at 4C. Predicted/Observed size: 15 kDa for Histone H3 K27Me3. Other band(s): none.
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Immunofluorescence of Histone H3 K27me3 Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 K27me3 antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 K27me3 is nuclear and chromosomal. Staining: Histone H3 K27me3 is expressed green and the nuclei are counterstained blue with DAPI.
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メーカー |
品番 |
包装 |
RKL
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600-401-J99
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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