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Immunofluorescence of Rabbit Anti-TACE Antibody. Cells: Rat Cortical Neurons. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells. (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity. (C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate. Double immunostaining of control and glutamate-exposed rat cortical cultures. (Hurtado et al., 2002).
Immunofluorescence of Rabbit Anti-TACE Antibody. Cells: Rat Brain. Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells. (Pradillo et al, 2005).
Immunofluorescence of Anti-TACE Antibody. Cells: HeLa Cells. Primary Antibody: Anti-TACE 10μg/mL. Secondary Antibody: Goat Anti-Rabbit IgG 1:500. Fixation: 4% paraformaldehyde.
Immunocytochemistry of Rabbit Anti-TACE Antibody. Cells: HeLa cells. Primary Antibody: Anti-TACE antibody at 10 μg/ml overnight at 2-8°C. Fixation: formaldehyde and blocked with 10% serum for 1 h at RT. Antigen Retrieval: heat mediation with a citrate buffer (pH6). Secondary Antibody: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Western Blot of Rabbit Anti-TACE Antibody. Lane (A, D): HeLa. Lane (B, E): Jurkat. Lane (C, F): Raji. Lanes A-C are in the absence or Lanes D-F are in the presence of blocking peptide. Primary Antibody: Anti-TACE at 1μg/mL RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Blocking 5% NFDM/TBST. Expect: ~93kDa.
Western Blot of Rabbit Anti-TACE Antibody. Loading: 15 μg of lysates per lane. Primary Antibodies: Anti-TACE 600-401-H24 (0.5 μg/mL), TACE 22001 (2 μg/mL), and GAPDH (0.02 μg/mL), for 1h incubation at RT. Block: 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000. Expect: ~93kDa.
Western Blot of Rabbit Anti-TACE Antibody. Monkey COS cells stably expressing Pref-1A were transfected with control siRNA or TACE siRNA. TACE was detected in lysates by using the anti-TACE antibody. TACE expression levels were markedly reduced in TACE knockdown cell lysate. (Wang et al., 2006).
Western Blot of Anti-TACE (ADAM-17) Antibody. ADAM-17 (TACE) protein expression, following transfection of vector and ADAM-17 cDNA, was examined by immunoblot analysis with anti-ADAM-17 antibodies in MCF-7 cells. Increased ADAM-17 was detected in ADAM-17 transfected cells. (McGowan et al., 2007).
Western Blot of Rabbit Anti-TACE (ADAM-17). ADAM-17 (TACE) protein expression in MDA-MB-435 Cells, following transfection with ADAM-17 shRNA (two clones) or neomycin-resistant negative control vector, was examined by immunoblot analysis with anti-ADAM-17 antibodies. (McGowan et al., 2007).
Western Blot of Anti-TACE Antibody. Effect of oxygen？glucose deprivation (OGD) or glutamate on the levels of TACE/ADAM17 in rat cortical cultures. (Hurtado et al., 2002). Western blot analysis of TACE in Rat Cortical Neuron homogenates from control, glutamate, and OGD-exposed cultures from a representative experiment.
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Immunofluorescence of Rabbit Anti-TACE Antibody. Cells: Rat Cortical Neurons. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells. (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity. (C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate. Double immunostaining of control and glutamate-exposed rat cortical cultures. (Hurtado et al., 2002).
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| 別品名 |
ADAM17, ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE
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| 交差種 |
Human
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 抗原部位 |
C-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
6868
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| Accession No.(Gene/Protein) |
NP_003174, P78536
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| Gene Symbol |
ADAM17
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| 参考文献 |
Black RA, Rauch CT , Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-α from cells. Nature 1997;385:729-733 Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-α. Nature 1997;385:733-736 Mizui Y, Yamazaki K, Sagane K, Tanaka I. cDNA cloning of mouse tumor necrosis factor-α converting enzyme (TACE) and partial analysis of its promoter. Gene 1999;233:67-74 Peschon JJ, Slack JL, Reddy P, et al. An essential role for ectodomain shedding in mammalian development. Science 1998;282:1281-4
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-H24
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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