Anti TACE

Immunocytochemistry of Rabbit Anti TACE Antibody. Cells: HeLa cells. Primary Antibody: Anti TACE antibody at 10 ug/ml overnight at 2 8C. Fixation: formaldehyde and blocked with 10% serum for 1 h at RT. Antigen Retrieval: heat mediation with a citrate buffer (pH6). Secondary Antibody: goat anti rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.

Immunofluorescence of Rabbit Anti-TACE Antibody. Cells: Rat Cortical Neurons. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells. (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity. (C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate. Double immunostaining of control and glutamate-exposed rat cortical cultures. (Hurtado et al., 2002).

Immunofluorescence of Rabbit Anti-TACE Antibody. Cells: Rat Brain. Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells. (Pradillo et al, 2005).

Immunofluorescence of Anti-TACE Antibody. Cells: HeLa Cells. Primary Antibody: Anti-TACE 10ug/mL. Secondary Antibody: Goat Anti-Rabbit IgG 1:500. Fixation: 4% paraformaldehyde.

Western Blot of Anti-TACE Antibody. Effect of oxygen？glucose deprivation (OGD) or glutamate on the levels of TACE/ADAM17 in rat cortical cultures. (Hurtado et al., 2002). Western blot analysis of TACE in Rat Cortical Neuron homogenates from control, glutamate, and OGD-exposed cultures from a representative experiment.

Western Blot of Anti-TACE (ADAM-17) Antibody. ADAM-17 (TACE) protein expression, following transfection of vector and ADAM-17 cDNA, was examined by immunoblot analysis with anti-ADAM-17 antibodies in MCF-7 cells. Increased ADAM-17 was detected in ADAM-17 transfected cells. (McGowan et al., 2007).

Western Blot of Rabbit Anti-TACE Antibody. Loading: 15 ug of lysates per lane. Primary Antibodies: Anti-TACE 600-401-H24 (0.5 ug/mL), TACE 22001 (2 ug/mL), and GAPDH (0.02 ug/mL), for 1h incubation at RT. Block: 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000. Expect: ~93kDa.

Western Blot of Rabbit Anti-TACE Antibody. Lane (A, D): HeLa. Lane (B, E): Jurkat. Lane (C, F): Raji. Lanes A-C are in the absence or Lanes D-F are in the presence of blocking peptide. Primary Antibody: Anti-TACE at 1ug/mL RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Blocking 5% NFDM/TBST. Expect: ~93kDa.

Western Blot of Rabbit Anti-TACE Antibody. Monkey COS cells stably expressing Pref-1A were transfected with control siRNA or TACE siRNA. TACE was detected in lysates by using the anti-TACE antibody. TACE expression levels were markedly reduced in TACE knockdown cell lysate. (Wang et al., 2006).

Western Blot of Rabbit Anti-TACE (ADAM-17). ADAM-17 (TACE) protein expression in MDA-MB-435 Cells, following transfection with ADAM-17 shRNA (two clones) or neomycin-resistant negative control vector, was examined by immunoblot analysis with anti-ADAM-17 antibodies. (McGowan et al., 2007).