|
※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry Validation of RIP3 in Mouse Lung.
Immunohistochemistry Validation of RIP3. Tissue: Mouse Thymus. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-RIP3 antibody at 1 μg/ml overnight at 4°C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Immunohistochemistry Validation of RIP3. Tissue: Rat Thymus. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-RIP3 antibody at 1 μg/ml overnight at 4°C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Immunohistochemistry of RIP3 antibody. Tissue: Rat kidney tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: RIP3 antibody at 5 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RIP3 is cytoplasmic and is also localized in the cell membrane and mitochondria. Staining: RIP3 as precipitated brown signal with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of RIP3 antibody. Tissue: Rat kidney cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: RIP3 antibody at 20 μg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RIP3 is cytoplasmic and is also localized in the cell membrane and mitochondria. Staining: RIP3 as red fluorescent signal.
Western Blot Validation of RIP3. Load: 15 μg of Rat Thymus lysate. Primary antibody: RIP3 at 0.5 μg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation of RIP3. Load: 15 μg of mouse C2C12 Cell lysates per lane. Primary antibody: RIP3 at (Lane 1: 0.1 μg/mL in the presence of peptide blocking. Lane 2: 0.1 μg/mL. Lane 3: 0.2 μg/mL. Lane 4: 0.5 μg/mL) for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation of RIP3. Load: 15 μg of mouse cell lysates. Lane 1: A20, Lane 2: C2C12, Lane 3: EL4, Lane 4: L1210, Lane 5: 3T3/NIH, Lane 6: BALB/3T3. Primary antibody: RIP3 at 0.5 μg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot of RIP3 antibody. Lane 1: Mouse 3T3 whole cell lysate. Lane 2: Mouse 3T3 whole cell lysate in the presence of blocking peptide. Load: 35 μg per lane. Primary antibody: RIP3 antibody at 1 μg/mL for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56.9 kDa, 62 kDa for RIP3. Other band(s):RIP3 splice variants and isoforms.
|
|
|
|
Immunohistochemistry Validation of RIP3 in Mouse Lung.
|
|
| 別品名 |
Receptor-interacting serine/threonine-protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, mRIP3, Ripk3, Rip3
|
| 交差種 |
Mouse
Rat
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunoprecipitation
|
| 免疫動物 |
Rabbit
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| GENE ID |
56532
|
| Accession No.(Gene/Protein) |
Q9QZL0
|
| Gene Symbol |
Ripk3
|
| 参考文献 |
Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP, Payan DG, Luo Y. Identification of RIP3, a RIP-like kinase that activates apoptosis and NFκB. Curr Biol. 1999;9(10):539-42. Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM. RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 1999;274(24):16871-5. Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-κB. Mol Cell Bio. 1999; 19(10):6500-8 (WD0102)
|
| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
600-401-H16
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
|
※当社では商品情報の適切な管理に努めておりますが、表示される法規制情報は最新でない可能性があります。
また法規制情報の表示が無いものは、必ずしも法規制に非該当であることを示すものではありません。
商品のお届け前に最新の製品法規制情報をお求めの際はこちらへお問い合わせください。
|
※当社取り扱いの試薬・機器製品および受託サービス・創薬支援サービス（納品物、解析データ等）は、研究用としてのみ販売しております。
人や動物の医療用・臨床診断用・食品用としては、使用しないように、十分ご注意ください。
法規制欄に体外診断用医薬品と記載のものは除きます。
|
|
※リンク先での文献等のダウンロードに際しましては、掲載元の規約遵守をお願いします。
|
