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Immunofluorescence Validation of NALP3. Cell: human K562 cells. Fixation: 4% paraformaldehyde-fixed.Primary Antibody: NALP3 at 5 μg/mL. Secondary: goat anti-rabbit IgG secondary antibody at 1:500 dilution (red) and DAPI staining (blue).
Immunofluorescence of NALP3. Cell: Human Molt4 cells. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: Anti-NALP3 at 20 μg/mL. Secondary: goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).
Immunohistochemistry of NALP3. Tissue: mouse thymus tissue. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6).Primary Antibody: anti-NALP3 antibody at 1 μg/mL overnight at 4°C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Immunohistochemistry of NALP3. Tissue: rat liver tissue: Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-NALP3 antibody at 1 μg/mL overnight at 4°C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Western Blot of NALP3. Loading: 15 μg of human, mouse and rat cell lysates. Lane 1: HeLa, Lane 2: HepG2, Lane 3: K562, Lane 4: MCF7, Lane 5: Jurkat, Lane 6: SK-N-SH, Lane 7: THP-1, Lane 8: 3T3/NIH, Lane 9: YB2/0. Primary antibody: Anti-NALP3 at 0.5 μg/mL for 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
Western Blot of NALP3. Load: 15 μg per lane. Lane 1: mouse spleen, Lane 2: mouse brain, Lane 3: mouse thymus. Primary antibody: Anti-NALP3 at 0.5 μg/mL for 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Western Blot of NALP3. ？Load: 15 μg per lane. Lane 1: rat liver, Lane 2: rat brain, Lane 3: rat thymus. Primary antibody: Anti-NALP3 at 0.5 μg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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Immunofluorescence Validation of NALP3. Cell: human K562 cells. Fixation: 4% paraformaldehyde-fixed.Primary Antibody: NALP3 at 5 μg/mL. Secondary: goat anti-rabbit IgG secondary antibody at 1:500 dilution (red) and DAPI staining (blue).
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| 別品名 |
NACHT, LRR and PYD domains-containing protein 3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, CLR1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, NLRP3, C1orf7, CIAS1, NALP3, PYPAF1
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| 交差種 |
Human
Mouse
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 抗原部位 |
N-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
114548
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| Accession No.(Gene/Protein) |
NP_001073289.1, Q96P20
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| Gene Symbol |
NLRP3
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| 参考文献 |
Chamaillard M, Girardin SE, Viala J, et al. Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation. Cell. Microbiol.2003; 5:581-92. O'Connor W Jr., Hartin JA, Zhu X, et al. Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties. J. Immunol.2003; 171:6329-33. Kanneganti TD, Ozoren N, Body-Malapal M, et al. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp. Nature2006; 440:233-6. Agostini L, Martinon F, Burns K, et al. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 2004; 20:319-25.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-H02
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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