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Western Blot Validation of Anti-DR5 in Human Cell Lines. Loading: 15 μg of lysates per lane. Lane 1: 293, Lane 2: A431, Lane 3: A549, Lane 4: CaCo-2, Lane 5: Daudi, Lane 6: HeLa, Lane 7: HepG2, Lane 8: K562, Lane 9: MCF-7, Lane 10: Jurkat, Lane 11: SK-N-SH, Lane 12: THP-1. Primary Antibody: Anti-DR5 at 0.5 μg/mL for 1h at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation of Anti-DR5. Load: 15 μg of Human HepG2 cells per lane. Primary Antibody: Anti-DR5 at [Lane 1: 1μg/mL, Lane 2: 2μg/mL, Lane 3: 4μg/mL] for 1h at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation of Anti-DR5.Loading: 15μg per lane. Lane 1: Mouse 3T3/NIH, Lane 2: Rat YB2/0. Antibodies: Anti-DR5 at 2μg/mL for 1h at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation of Anti-DR5. Loading: 15 μg of lysates per lane. Lane 1: Mouse 3T3/NIH, Lane 2: C2C12.Primary Antibody: Anti-DR5 at 1μg/mL for 1h at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation of Anti-DR5. Loading: 15 μg of Mouse Heart lysate. Primary Antibody: Anti-DR5 at 1μg/mL at 1h at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation of Anti-DR5. Loading: 15 μg of Rat Skeletal Muscle lysate. Primary Antibody: Anti-DR5 1μg/mL for 1h at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of DR5. Tissue: Mouse kidney tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-DR5 antibody at 5μg/ml overnight at 4°C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Immunofluorescence Validation of DR5.Cells: Human HepG2 Cells. Fixation: 4% paraformaldehyde-fixed. PRimary Antibody: Anti-DR5 at 5 μg/mL. Secondary: goat anti-rabbit IgG antibody at 1:500 dilution (green) and DAPI (blue).
Immunofluorescence of Anti-DR5Cells: Human Testis. Fixation: 4% paraformaldehyde-fixed. Priamry Antibody: Anti-DR5 at 10 μg/mLSecondary: goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI (blue).
Immunofluorescence Validation of DR5. Tissue: Mouse Pancreas. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: Anti-DR5 at 10 μg/mL. Secondary: goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI (blue).
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Western Blot Validation of Anti-DR5 in Human Cell Lines. Loading: 15 μg of lysates per lane. Lane 1: 293, Lane 2: A431, Lane 3: A549, Lane 4: CaCo-2, Lane 5: Daudi, Lane 6: HeLa, Lane 7: HepG2, Lane 8: K562, Lane 9: MCF-7, Lane 10: Jurkat, Lane 11: SK-N-SH, Lane 12: THP-1. Primary Antibody: Anti-DR5 at 0.5 μg/mL for 1h at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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| 別品名 |
DR5, KILLER, TRAILR2, TRICK2, ZTNFR9
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 抗原部位 |
C-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
8795
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| Accession No.(Gene/Protein) |
NP_003833.4, O14763
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| Gene Symbol |
TNFRSF10B
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| 参考文献 |
Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8 Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21 Walczak H, Degli-Esposti MA, Johnson RS, Smolak PJ, Waugh JY, Boiani N, Timour MS, Gerhart MJ, Schooley KA, Smith CA, Goodwin RG, Rauch CT. TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL. EMBO J 1997;16:5386-97 MacFarlane M, Ahmad M, Srinivasula SM, Fernandes-Alnemri T, Cohen GM, Alnemri ES. Identification and molecular cloning of two novel receptors for the cytotoxic ligand TRAIL. J Biol Chem 1997;272:25417-20
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-G96
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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