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Western Blot of ACE2 Antibody. Lane 1: Mouse Liver Lysate. Lane 2: Mouse Spleen Lysate. Lane 3: Mouse Heart Lysate. Lane 4: Mouse Bladder Lysate. Lane 5: Mouse Pancreas Lysate. Lane 6: Mouse Stomach Lysate. Loading: 15 μg of lysates per lane. Primary Antibody: Anti ACE2 at 2μg/mL for 1hr at RT in 5% BLOTTO/TBST. Secondary Antibody: Goat anti rabbit IgG HRP conjugate at 1:10000 dilution. Predicted MW: ~93kDa. Observed MW: ~125kDa.
Immunofluorescene of Rabbit Anti-ACE2 Antibody. Tissue: Human Lung Tissue. Fixative: 4% PFA. Primary Antibody: Anti-ACE2 at 20μg/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunohistochemistry of Anti-ACE2 Antibody. Tissue: Human Kidney Tissue. Fixation: formaldahyde and blocked with 10% serum for 1hr at RT. Antigen retrieval: heat mediation with Citrate Buffer pH6. Primary antibody: ACE2 antibody at 2ug/mL for overnight at 2-8C. Secondary antibody: Goat Anti-rabbit HRP secondary antibody 1:250 for 45 min at RT. Counterstain: hematoxylin.
Immunofluorescence of Rb Anti-ACE2 Antibody. Tissue: Mouse Lung Tissue. Fixative: 4% PFA. Primary Antibody: Anti-ACE2 at 20μg/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Rat Lung Tissue. Fixative: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Rabbit Anti-ACE2 Antibody. Tissue: Caco2 Cells. Fixative: 4%PFA. Primary Antibody: Anti-ACE2 at 5ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue). Image showing membrane staining on Caco2 cells.
Immunofluorescence of Rabbit Anti-ACE2 Antibody. Tissue: Human Testis. Fixative: 4% PFA. Primary Antibody: Anti-ACE2 at 20μg/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence Microscopy of Rabbit Anti-ACE2 antibody. Tissue: Human Kidney Tissue. Fixation: 4% PFA. Primary antibody: ACE2 antibody at 10 ug/mL for overnight at 2-8C. Secondary antibody: Goat Anti-Rabbit IgG Conjugated 1:500 for 1hr at RT.
Western Blot of Rabbit Anti-ACE2 Antibody. Load: Human Kidney Lysate. Primary Antibody at - Lane A: 0.5ug/mL, Lane B: 1.0ug/mL, Lane C: 2.0 ug/mL. Expect: ~92kDa.
Western Blot of different Rabbit anti-ACE2 antibodies. Lane 1: Human Kidney Lysate. Lane 2: Human Spleen Lysate. Lane 3: Human Testis Lysate. Lane 4: Human Breast Lysate. Lane 5: Human Intestine Lysate. Load: 15 ug per lane. Primary antibody: ACE2 antibody (600-401-X58, 600-401-G71, 600-401-X59) at 2ug/mL and Beta Actin at 1ug/mL for 1hr at RT. Secondary antibody: Goat anti-Rabbit secondary HRP antibody. Block: 5% BLOTTO/TBST. Predicted MW: ~93kDa. Observed: ~130kDa.
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