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Immunofluorescence Microscopy of Rabbit Anti-ACE2 antibody. Tissue: Human Kidney Tissue. Fixation: 4% PFA. Primary antibody: ACE2 antibody at 10 μg/mL for overnight at 2-8°C. Secondary antibody: Goat Anti-Rabbit IgG Conjugated 1:500 for 1hr at RT.
Immunofluorescence of Rabbit Anti-ACE2 Antibody. Tissue: Human Testis. Fixative: 4% PFA. Primary Antibody: Anti-ACE2 at 20μg/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescene of Rabbit Anti-ACE2 Antibody. Tissue: Human Lung Tissue. Fixative: 4% PFA. Primary Antibody: Anti-ACE2 at 20μg/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Rb Anti-ACE2 Antibody. Tissue: Mouse Lung Tissue. Fixative: 4% PFA. Primary Antibody: Anti-ACE2 at 20μg/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Rat Lung Tissue. Fixative: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Rabbit Anti-ACE2 Antibody. Tissue: Caco2 Cells. Fixative: 4%PFA. Primary Antibody: Anti-ACE2 at 5ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue). Image showing membrane staining on Caco2 cells.
Immunohistochemistry of Anti-ACE2 Antibody. Tissue: Human Kidney Tissue. Fixation: formaldahyde and blocked with 10% serum for 1hr at RT. Antigen retrieval: heat mediation with Citrate Buffer pH6. Primary antibody: ACE2 antibody at 2μg/mL for overnight at 2-8°C. Secondary antibody: Goat Anti-rabbit HRP secondary antibody 1:250 for 45 min at RT. Counterstain: hematoxylin.
Western Blot of different Rabbit anti-ACE2 antibodies. Lane 1: Human Kidney Lysate. Lane 2: Human Spleen Lysate. Lane 3: Human Testis Lysate. Lane 4: Human Breast Lysate. Lane 5: Human Intestine Lysate. Load: 15 μg per lane. Primary antibody: ACE2 antibody (600-401-X58, 600-401-G71, 600-401-X59) at 2μg/mL and Beta Actin at 1μg/mL for 1hr at RT. Secondary antibody: Goat anti-Rabbit secondary HRP antibody. Block: 5% BLOTTO/TBST. Predicted MW: ~93kDa. Observed: ~130kDa.
Western Blot of Rabbit Anti-ACE2 Antibody. Lane 1: Human Testis Lysate. Lane 2: Human Lung Lysate. Lane 3: Human Intestine Lysate. Lane 4: Human Breast Lysate. Lane 5: Caco2 Lysate. Loading: 15 μg of lysates per lane. Primary Antibody: Anti-ACE2 at 2μg/mL for 1hr at RT in 5% BLOTTO/TBST. Secondary Antibody: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted MW: ~93kDa. Observed MW: ~130kDa.
Western Blot of ACE2 Antibody. Lane 1: Mouse Liver Lysate. Lane 2: Mouse Spleen Lysate. Lane 3: Mouse Heart Lysate. Lane 4: Mouse Bladder Lysate. Lane 5: Mouse Pancreas Lysate. Lane 6: Mouse Stomach Lysate. Loading: 15 μg of lysates per lane. Primary Antibody: Anti-ACE2 at 2μg/mL for 1hr at RT in 5% BLOTTO/TBST. Secondary Antibody: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted MW: ~93kDa. Observed MW: ~125kDa.
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Immunofluorescence Microscopy of Rabbit Anti-ACE2 antibody. Tissue: Human Kidney Tissue. Fixation: 4% PFA. Primary antibody: ACE2 antibody at 10 μg/mL for overnight at 2-8°C. Secondary antibody: Goat Anti-Rabbit IgG Conjugated 1:500 for 1hr at RT.
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| 抗原部位 |
C-terminus
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
Mouse
Rat
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| GENE ID |
59272
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| Accession No.(Gene/Protein) |
NP_068576, Q9BYF1
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| Gene Symbol |
ACE2
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotension I to angiotension 1-9. Circ. Res. 2000;87:1-9. Tipnis SR, Hooper NM, Hyde R, et al. A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol. Chem. 2000;275:33238-43. Li W, Moore MJ, Vasileva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003;426:450-4. Crackower MA, Sarao R, Oudit GY, et al. Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature 2002;417:822-8.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-G71
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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