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Immunofluorescence Microscopy of Rabbit Anti-ACE2 antibody.
Tissue: Human Kidney Tissue.
Fixation: 4% PFA.
Primary antibody: ACE2 antibody at 10 μg/mL for overnight at 2-8C.
Secondary antibody: Goat Anti-Rabbit IgG Conjugated 1:500 for 1hr at RT.
Immunofluorescence of Rabbit Anti-ACE2 Antibody.
Tissue: Human Testis.
Fixative: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescene of Rabbit Anti-ACE2 Antibody.
Tissue: Human Lung Tissue.
Fixative: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain
(blue).
Immunofluorescence of Rb Anti-ACE2 Antibody.
Tissue: Mouse Lung Tissue.
Fixative: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody.
Tissue: Rat Lung Tissue.
Fixative: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary antibody at
1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Rabbit Anti-ACE2 Antibody.
Tissue: Caco2 Cells.
Fixative: 4%PFA.
Primary Antibody: Anti-ACE2 at 5μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary antibody at
1:500 dilution (green) and DAPI counterstain (blue). Image
showing membrane staining on Caco2 cells.
Immunohistochemistry of Anti-ACE2 Antibody.
Tissue: Human Kidney Tissue.
Fixation: formaldahyde and blocked with 10% serum for 1hr at RT.
Antigen retrieval: heat mediation with Citrate Buffer pH6.
Primary antibody: ACE2 antibody at 2μg/mL for overnight at 2-8C.
Secondary antibody: Goat Anti-rabbit HRP secondary antibody 1:250 for 45 min at RT.
Counterstain: hematoxylin.
Western Blot of different Rabbit anti-ACE2 antibodies. Lane 1: Human Kidney Lysate. Lane 2: Human Spleen Lysate. Lane 3: Human Testis Lysate. Lane 4: Human Breast Lysate. Lane 5: Human Intestine Lysate. Load: 15 μg per lane. Primary antibody: ACE2 antibody (600-401-X58, 600-401-G71, 600-401-X59) at 2μg/mL and Beta Actin at 1μg/mL for 1hr at RT. Secondary antibody: Goat anti-Rabbit secondary HRP antibody. Block: 5% BLOTTO/TBST. Predicted MW: ~93kDa. Observed: ~130kDa.
Western Blot of Rabbit Anti-ACE2 Antibody.
Lane 1: Human Testis Lysate.
Lane 2: Human Lung Lysate.
Lane 3: Human Intestine Lysate.
Lane 4: Human Breast Lysate.
Lane 5: Caco2 Lysate.
Loading: 15 μg of lysates per lane.
Primary Antibody: Anti-ACE2 at 2μg/mL for 1hr at RT in 5% BLOTTO/TBST.
Secondary Antibody: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Predicted MW: ~93kDa.
Observed MW: ~130kDa.
Western Blot of ACE2 Antibody.
Lane 1: Mouse Liver Lysate.
Lane 2: Mouse Spleen Lysate.
Lane 3: Mouse Heart Lysate.
Lane 4: Mouse Bladder Lysate.
Lane 5: Mouse Pancreas Lysate.
Lane 6: Mouse Stomach Lysate.
Loading: 15 μg of lysates per lane.
Primary Antibody: Anti-ACE2 at 2μg/mL for 1hr at RT in 5% BLOTTO/TBST.
Secondary Antibody: Goat anti-rabbit IgG HRP conjugate at 1:10000
dilution.
Predicted MW: ~93kDa.
Observed MW: ~125kDa.
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