| 抗原部位 |
a.a.117-380
|
| 種由来 |
Human
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Immunohistochemistry
|
| 免疫動物 |
Mouse
|
| 抗体クラス |
IgG1
|
| クローン |
OTI3A8
|
| 交差種 |
Human
Mouse
Rat
|
| Accession No.(Gene/Protein) |
NM_004111, P39748
|
| Gene Symbol |
FEN1
|
| 分子量 |
42.4 kDa
|
| 使用目的 |
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008].
|
| その他 |
[locus ID]2237
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN1 (RC201785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FEN1. Positive lysates LY401328 (100ug) and LC401328 (20ug) can be purchased separately from OriGene.
|
|
|
|
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN1 (RC201785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FEN1. Positive lysates LY401328 (100ug) and LC401328 (20ug) can be purchased separately from OriGene.
|
|
|
| メーカー |
品番 |
包装 |
|
ORG
|
TA802764
|
100 UL
[1.00 mg/ml]
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
|
