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The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: NIH3T3 cell lysate
Lane 4.: Mouse brain tissue lysate
Lane 5.: Mouse liver tissue lysate
The tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Kidney tissue lysate
ICC/IF analysis of PARK7 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of PARK7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: NIH3T3 cell lysate
Lane 4.: Mouse brain tissue lysate
Lane 5.: Mouse liver tissue lysate
The tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Kidney tissue lysate
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| 別品名 |
Parkinson disease protein 7, PARK7, DJ-1 (PARK7), Oncogene DJ1, Parkinson disease protein 7, Protein DJ-1, Parkinson disease protein 7 CAP1, DJ1, DJ1 protein, Oncogene DJ1, SP22.
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
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| 免疫動物 |
Mouse
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| クローン |
AT1E12
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| 抗体クラス |
IgG2bκ
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| 抗原部位 |
a.a.1-189
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| Accession No.(Gene/Protein) |
NP_009193, Q99497
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| 概要 |
Parkinson disease (autosomal recessive, early onset) 7, also known as PARK7/DJ-1, has been shown to interact with EFCAB6 and protein inhibitor of activated STAT2. Defects in PARK7 are the cause of autosomal recessive early-onset Parkinson's disease 7. This protein belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death.
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| 参考文献 |
Entrez Gene: PARK7
Bonifati V., et al. (2003) Science 299 (5604): 256-259
Takahashi K., et al. (2001) J. Biol. Chem. 276 (40): 37556-63
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| メーカー |
品番 |
包装 |
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ATG
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ATGA0292
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50 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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