| 別品名 |
Abeta, Amyloid beta, Beta Amyloid peptide, Beta Amyloid Antibody
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| 抗原部位 |
N-terminus
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Dot Blot
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 交差種 |
Human
|
| GENE ID |
351
|
| Accession No.(Gene/Protein) |
NP_000475, P05067
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| Gene Symbol |
APP
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Schilling S, Lauber T, Schaupp M, Manhart S, Scheel E, Bohm G, Demuth HU. On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry;45(41):12393-9. 2006 Oct 17. [PMID: 17029395] He W, Barrow CJ. The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry;38(33):10871-7 . 1999 Aug 17. [PMID: 10451383]
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence Microscopy of Rabbit Anti beta Amyloid pyro E3 antibody. Tissue: human brain section. Fixation: .5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:1, for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: B Amyloid pyro E3 as green fluorescent signal, B Amyloid 3 as yellow signal; and Bottom: B Amyloid 3 as red signal with co incubation of B Amyloid pyro E3 peptide.
Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody. Lane 1: beta Amyloid 3 peptide Lane 2: beta Amyloid pyro E3 peptide. Load: 1 μg per lane. Primary antibody: s Amyloid pyro E3 antibody at 2μg/mL for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide.
Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody. Lane 1-1: beta Amyloid pyro E3 peptide. Load: 1 ug per lane 1-2, .2 ug per lane 3-4, .5 ug per lane 5-6, .1 ug per lane 7-8, .5 ug per lane 9-1. Primary antibody: B Amyloid pyro E3 antibody at .5 ug/mL for overnight at 4C. Secondary antibody: IRDye8TM rabbit secondary antibody at 1:1, for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide. Cross reactivity with B Amyloid 3 peptide is >1 fold below pyrE3 reactivity and contains no reactivity towards the B Amyloid 42 Aβ peptide (data not shown).
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Immunofluorescence Microscopy of Rabbit Anti beta Amyloid pyro E3 antibody. Tissue: human brain section. Fixation: .5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:1, for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: B Amyloid pyro E3 as green fluorescent signal, B Amyloid 3 as yellow signal; and Bottom: B Amyloid 3 as red signal with co incubation of B Amyloid pyro E3 peptide.
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| メーカー |
品番 |
包装 |
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RKL
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600-401-J14
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50 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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