Anti β Amyloid pyro E3  

Immunofluorescence Microscopy of Rabbit Anti-beta Amyloid pyro E3 antibody. Tissue: human brain section. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 μg/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: s Amyloid pyro E3 as green fluorescent signal, s Amyloid 3 as yellow signal; and Bottom: s Amyloid 3 as red signal with co-incubation of s Amyloid pyro E3 peptide.

Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody. Lane 1: beta Amyloid 3 peptide Lane 2: beta Amyloid pyro E3 peptide. Load: 1 μg per lane. Primary antibody: s Amyloid pyro E3 antibody at 2μg/mL for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide.

Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody. Lane 1-10: beta Amyloid pyro E3 peptide. Load: 1 μg per lane 1-2, 0.2 μg per lane 3-4, 0.05 μg per lane 5-6, 0.01 μg per lane 7-8, 0.005 μg per lane 9-10. Primary antibody: s Amyloid pyro E3 antibody at 0.5 μg/mL for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide. Cross reactivity with s Amyloid 3 peptide is >1000 fold below pyrE3 reactivity and contains no reactivity towards the s Amyloid 42 Aβ peptide (data not shown).