| 別品名 |
AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
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| 免疫動物 |
Mouse
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| 抗体クラス |
IgG1κ
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| クローン |
9A5.H9.G7
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| 交差種 |
Human
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| GENE ID |
10000
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| Accession No.(Gene/Protein) |
NP_001193658, Q9Y243
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| Gene Symbol |
AKT3
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Madhunapantula SV, et al.The Akt signaling pathway: An emerging therapeutic target in malignant melanoma. Cancer Biol Ther. 2011 Dec 15;12(12) Gonzalez E, McGraw TE. The Akt kinases: isoform specificity in metabolism and cancer.Cell Cycle. 2009 Aug 15;8(16):2502-8 Madhunapantula SV, Robertson GP. The PTEN-AKT3 signaling cascade as a therapeutic target in melanoma. Pigment Cell Melanoma Res. 2009 Aug;22(4):400-19.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA of Mouse Monoclonal anti AKT3 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti AKT3 antibody at 100ng/well. Dilution series: 2 fold. Mid point concentration: 3 ng/mL Mouse monoclonal anti AKT3 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE 0100).
Immunofluorescence of Mouse monoclonal anti-AKT3 antibody. Cell Type: A431 cells. Fixation: 4% paraformaldehyde 10 min. Permeabilization: 0.5% Triton X 30 min. Primary Ab: (p/n 200-301-J36) at 1:250 for 72 hours 4°C. Secondary Ab: (p/n 610-142-121) at 1:1000 overnight 4°C.
Western Blot of Mouse monoclonal anti-AKT3 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Lane 4: Molecular Weight Markers. Load: 50 ng per lane. Primary antibody: anti-AKT3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Rabbit secondary antibody anti mouse at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 85 kDa for GST-AKT3.
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ELISA of Mouse Monoclonal anti AKT3 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti AKT3 antibody at 100ng/well. Dilution series: 2 fold. Mid point concentration: 3 ng/mL Mouse monoclonal anti AKT3 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE 0100).
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| メーカー |
品番 |
包装 |
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RKL
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200-301-J36
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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