| 別品名 |
synthetic FLAG(R) peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide
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| 適用 |
Western Blot
Immunoprecipitation
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| タグ（タンパク質） |
FLAG
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| 形状 |
凍結乾燥品
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| 参考文献 |
A. Einhauer and A. Jungbauer, (2001), The FLAG(R) peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochemical Biophysical Methods, 49 (1); 455-465.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
SDS PAGE of Anti DYKDDDDK (FLAGR tag) Affinity Gel. Lane 1: Cell lysate before purification. Lane 2: Flow through (used cell lysate). Lane 3: Purified DYKDDDDK (FLAGR tag) recombinant protein (arrowhead). Load: (6 uL per lane). Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.
SDS-PAGE of Anti-DYKDDDDK affinity gel using DYKDDDDK control peptide. Lane 1: DYKDDDDK tagged recombinant protein. Lane 2: flow through. Lane 3: wash. Lane 4: eluted fraction 1. Lane 5: eluted fraction 2. Lane 6: eluted fraction 3. Lane 7: blank. Lane 8: molecular weight markers. Load: 5 μg protein lane 1, 15μL per each other lane. Predicted/Observed size: 135kDa for DYKDDDDK tagged recombinant protein.
SDS-PAGE of Anti-DYKDDDDK (FLAGR tag) Affinity Gel. Lane 1: crude lysate containing over-expressed DYKDDDK-tagged recombinant protein . Lane 2: unbound flow-through of consisting of endogenous E.coli proteins. Lane 3: enriched recombinant protein. Load: 5 ug protein, 15uL flow through. Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.
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SDS PAGE of Anti DYKDDDDK (FLAGR tag) Affinity Gel. Lane 1: Cell lysate before purification. Lane 2: Flow through (used cell lysate). Lane 3: Purified DYKDDDDK (FLAGR tag) recombinant protein (arrowhead). Load: (6 uL per lane). Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.
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| メーカー |
品番 |
包装 |
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RKL
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000-000-383
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1 MG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃
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