別品名 |
L-lactate dehydrogenase A chain LDH-A LDH muscle subunit LDH-M
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種由来 |
Rabbit
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標識物 |
Biotin
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精製度 |
Ig fraction - Ion Exchange /Gel Filtration
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immuno Fluorescence
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免疫動物 |
Goat
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交差種 |
Rabbit
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GENE ID |
100009107
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Accession No.(Gene/Protein) |
P13491
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Gene Symbol |
LDHA
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形状 |
凍結乾燥品
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参考文献 |
[Pub Med ID]26837579
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence Microscopy of Biotin conjugated Anti Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB 071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.
Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 μg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800? secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
Western Blot of Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.
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Immunofluorescence Microscopy of Biotin conjugated Anti Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB 071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.
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メーカー |
品番 |
包装 |
RKL
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200-1673-0100
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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法規制 |
毒
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保存温度 |
4℃
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