Anti Histone H3 K36ac  

Dot Blot of Rabbit Anti-Histone H3 [Lys36 ac] Antibody. Lane 1: K36. Lane 2: K36-KMe2. Lane 3: K36-KAc. Lane 4: K36-KMe1. Load: 0.03, 0.1, 0.3, 1.0, 3.0 μg of peptide. Primary antibody: Histone H3 [Lys36 ac] for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO A overnight at 4°C.

Western Blot of Rabbit Anti-Histone H3 [ac Lys36] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody: Histone H3 [ac Lys36] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Immunofluorescence of Anti-Histone H3 K36ac: Histone H3 Ac Lys36 antibody was tested at 1:200,000 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X)