Anti Histone H3 K36ac  

Dot Blot of Rabbit Anti-Histone H3 [Lys36 ac] Antibody. Lane 1: K36. Lane 2: K36-KMe2. Lane 3: K36-KAc. Lane 4: K36-KMe1. Load: 0.03, 0.1, 0.3, 1.0, 3.0 μg of peptide. Primary antibody: Histone H3 [Lys36 ac] for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO A overnight at 4°C.

Western Blot of Rabbit Anti-Histone H3 [ac Lys36] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody: Histone H3 [ac Lys36] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800^TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Immunofluorescence of Anti-Histone H3 K36ac: Histone H3 Ac Lys36 antibody was tested at 1:200,000 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X)

C) Western blot analysis of specificity of H3K36ac antibodies using WT and H3-K36A chromatin extracts (p/n 600？401-I89). Total H4 and H3 were used as loading controls. Fig 1. PMID:？33522486

A) Slot blot analysis to quantitatively assess whether anti-K36 acetyl antibodies equivalently recognize WT, H3-G34R, or H3-G34K peptides bearing H3K36ac modification. Peptides were loaded in twofold serial dilution. Rockland p/n 600？401-I89; Ponceau-stained blots were used as the loading control (left). Fig 5. PMID:？33522486