Anti H2AX, phospho Ser139

Western Blot of Rabbit anti H2AXpS139 antibody. Lane 1: HeLa Lysate stimulated with adriamycin (24 hr). Lane 2: HeLa Lysate unstimulated. Load: 35 ug per lane. Primary antibody: H2AXpS139 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band(s): none.

Western Blot of Rabbit Anti-H2AX pS139 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HEK293T Whole cell Lysate (p/n W09-001-GX5) [+]. Lane 3: MCF-7 Whole cell Lysate (p/n W09-000-360) [+]. Lane 4: U-87-MG Whole cell Lysate (p/n W09-001-GX2) [-]. Primary Antibody: Anti-H2AXpS139 at 5ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Universal Fluorescent Buffer (p/n MB-070) for 1hr at RT. Predicted MW: ~15kDa. Observed MW: ~17kDa. Exposure: 10sec.