Anti TLR3  

Flow Cytometry of Mouse Anti-TLR3 antibody. Cells: Human monocytes. Stimulation: none. Primary Antibody: Anti-TLR3 antibody at 0.5 ug (red) and isotype control (green). Secondary Antibody: Goat anti-mouse IgG1 PE conjugate (BD).

Immunohistochemistry of mouse Anti-TLR3 antibody. Tissue A: Human gut lumen (longitudinal section, tranverse region) using TLR3. Tissue B: Mouse spleen tissue using isotype control. Tissue C: Mouse spleen tissue using TLR3. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: TLR3 antibody at tissue A at 10 μg/mL and at tissue C at 5 mg/ml for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: TLR3 is an endoplasmic reticulum membrane and a single-pass type 1 membrane protein. Staining: TLR3 is precipitated as a red signal with hematoxylin purple nuclear counterstain.

Western Blot of Mouse Anti-TLR3 antibody. Lane A: untransfected 293. Lane B: 293 cells with human TLR3 cDNA. Lane C: human intestine. Lane D: placenta. Lane E: heart. Lane F: ovary. Primary antibody: TLR3 antibody at 3 μg/mL for overnight at 4°C. Secondary antibody: Goat anti-mouse HRP conjugate at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80 kDa for TLR3. Other band(s): none.