Anti Caspase-8  

Immunohistochemistry of mouse Anti-Caspase-8 antibody. Tissue: Human stomach tumor. Fixation: Formalin fixed paraffin embedded. Antigen retrieval: Not required. Primary antibody: Caspase-8 at 5 μg/ml for 1 h at RT (bottom left/right). Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Top left: Unstained isotype control. Bottom left, right: Caspase-8 is stained brown.

Western Blot of Mouse Anti-Caspase-8 antibody. Lane 1-5: Jurkat cells lysate. Treated with 2 μM of staurosporine for 0, 0.5, 1, 2, 4 hours respectively. Primary antibody: Caspase-8 antibody at 1 μg/ml for overnight at 4°C. Secondary antibody: IRDye800？ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55.4 kDa for Pro-Caspase-8. Other band(s): p18, p41/p43 cleavage fragments.

Flow Cytometry of Mouse Anti-Caspase-8 antibody. Cells: HeLa cells. Stimulation: none. Primary Antibody: Caspase-8 antibody at 0.1 μg/mL (red) and isotype control (green). Secondary Antibody: Goat anti-mouse IgG-FITC.