| 別品名 |
Activating transcription factor 6 alpha
|
| 抗原部位 |
N-terminus
|
| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Ig fraction - Protein G
|
| 適用 |
Western Blot
Immunohistochemistry
Immuno Fluorescence
Flow Cytometry
Immunoprecipitation
Chromatin Immunoprecipitation
|
| 免疫動物 |
Mouse
|
| 抗体クラス |
IgG1κ
|
| クローン |
70B1413.1
|
| 交差種 |
Human
Mouse
|
| GENE ID |
22926
|
| Accession No.(Gene/Protein) |
NP_031374, P18850
|
| Gene Symbol |
ATF6
|
| 形状 |
液状
|
| 参考文献 |
1. Groenendyk J and M Michalak. 2005. Endoplasmic reticulum quality control and apoptosis. Acta Biochimica Polonica 52:381-295. 2. Shen J and R Prywes. 2005. ER stress signaling by regulated proteolysis of ATF6. Methods 35:382-389.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Mouse Anti Androgen Receptor antibody. Lane A: LNCaP untreated cells. Lane B: LNCaP cells treated with 100ng IGF 1 for 4hrs. Lane C: LNCaP cells incubated with 20um LY294002 for 30min prior to treatment with 100ng IGF 1 for 4hrs. Load: 30 ug per lane. Primary antibody: Androgen Receptor antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800TM mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~110 kDa for Androgen Receptor antibody. Other band(s): none.
Western Blot of Mouse Anti-ATF6 antibody. Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6. Lane 3: Untransfected 293 cells. Load: 30 μg per lane. Primary antibody: ATF6 antibody at 4 μg/ml for overnight at 4°C. Secondary antibody: IRDye800？ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~90 kDa for ATF6. Other band(s): none.
Western Blot of Mouse Anti-ATF6 antibody. Lane A: NIH3T3 lysate. Load: 30 ug per lane. Primary antibody: ATF6 antibody at 3 ug/ml for overnight at 4C. Secondary antibody: IRDye800TM mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~90 kDa for ATF6 antibody. Other band(s): possible breakdown/cleavage product.
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Western Blot of Mouse Anti Androgen Receptor antibody. Lane A: LNCaP untreated cells. Lane B: LNCaP cells treated with 100ng IGF 1 for 4hrs. Lane C: LNCaP cells incubated with 20um LY294002 for 30min prior to treatment with 100ng IGF 1 for 4hrs. Load: 30 ug per lane. Primary antibody: Androgen Receptor antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800TM mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~110 kDa for Androgen Receptor antibody. Other band(s): none.
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|
| メーカー |
品番 |
包装 |
|
RKL
|
200-301-H56
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
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