| 別品名 |
H4/A, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, H4/M, H4/N, H4/O, H4/p, H4F2, H4FA, H4FB, H4FC, H4FD, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4, histone H4, MGC24116
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| 抗原部位 |
N-terminus
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Immuno Fluorescence
Chromatin Immunoprecipitation
Dot Blot
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 交差種 |
Human
Caenorhabditis elegans
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| 翻訳後修飾 |
メチル化
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| GENE ID |
121504
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| Accession No.(Gene/Protein) |
NP_001029249, P62805
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| Gene Symbol |
HIST4H4
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Campsteijn C., OvrebO JI., Karlsen BO., Thompson EM. (2012). Expansion of cyclin D and CDK1 paralogs in Oikopleura dioica, a chordate employing diverse cell cycle variants. Mol. Biol. Evol. 2012 Feb. 29:487-502. Yue C., Soboloff J., Gamero AM. (2012). Control of type I interferon-induced cell seath by Orai1-mediated calcium entry in T cells. J Biol Chem. 2012 Jan. 287:3207-3216. Kung VL., Khare S., Stehlik C., Bacon EM., Hughes AJ., Hauser AR. (2012). An rhs gene of Pseudomonas aeruginosa encodes a virulence protein that activates the inflammasome. Proc Natl Acad Sci USA. 2012 Jan. 109:1275-1280. Sullivan CP., Berg EA., Elliot-Bryant R., Fishman JB., Mckee AC., Morin PJ., et al. (2011). Pyroglutamate-Αβ 3 and 11 colocalize in amyloid plaques in Alzheimer's disease cerebral cortex with pyroglutamate-Αβ 11 forming the central core. Neurosci Lett. 2011 Nov 14. 505(2):109-12. Daftarian P., Kaifer AE., Li W., Blomberg BB., Frasca D., Roth F., et al. (2011). Peptide-conjugated PAMAM dendrimer as a universal platform for antigen presenting cell targeting and effective DNA-based vaccinations. Cancer Res. 2011 Oct 10. 71(24):7452-62.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Dot Blot of Rabbit Histone H4 [Dimethyl Lys20] Antibody. Lane 1: K9 K14. Lane 2: K9 Kac K14 Kac. Lane 3: K9 K14 Kac. Lane 4: K9 ac K14. Load: 0.3, 1, 3, 10, and 30 picomoles of peptide. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at 1:2000 for 45 min at 4C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
Immunofluorescence of Rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Dimethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Dimethyl Lys20] is expressed in green, nuclei are counterstained with DAPI (blue).
Chromatin Immunoprecipitation of Rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H4 K20me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Western Blot of Rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 ug per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~13 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: HeLa histone preps. Load: 30 ug per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~13 kDa. Other band(s): None.
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Dot Blot of Rabbit Histone H4 [Dimethyl Lys20] Antibody. Lane 1: K9 K14. Lane 2: K9 Kac K14 Kac. Lane 3: K9 K14 Kac. Lane 4: K9 ac K14. Load: 0.3, 1, 3, 10, and 30 picomoles of peptide. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at 1:2000 for 45 min at 4C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
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| メーカー |
品番 |
包装 |
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RKL
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600-401-J02
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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