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Immunofluorescence of Rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Lys20] antibody at a 1:500 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Monomethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Lys20] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot of Rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody: Histone H4 [Monomethyl Lys20] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
Chromatin Immunoprecipitation Rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H4K20me1 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Western Blot of Rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 μg per lane. Primary antibody: Histone H4 [Monomethyl Lys20] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
Dot Blot of Rabbit Histone H4 [Monomethyl Lys20] Antibody. Antigen: Lane 1: unmodified. Lane 2: K20me1. Lane 3: K20me2. Lane 4: K20me3. Load: 0.6, 1, 3, 6, and 10 picomoles of peptide. Primary antibody: Histone H4 [Monomethyl Lys20] antibody at 2 μg/ml for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
？(d,？e)？293T cells were treated with AZD6738 or DMSO for 30？min and then fractionated. Nuclease-insoluble fraction was analyzed by mass spectrometry showing increase of the proteins involved in replication. Western blots confirm the MS results.？While ATR and RPA34 accumulate in the chromatin after treatments with DNA damaging agents, neither of these proteins were recruited to the chromatin in cells treated with ATRi alone in Fig.？1e. [p/n 600-401-I72 H3, acK9; p/n 600-401-J01, H4 meK20) Fig 1. PMID:？29123096
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Immunofluorescence of Rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Lys20] antibody at a 1:500 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Monomethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Lys20] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
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| 別品名 |
rabbit anti-Histone H4 monomethyl Lys20 antibody, H4K20me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
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| 交差種 |
Human
Mouse
Caenorhabditis elegans
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| 適用 |
Western Blot
Immuno Fluorescence
Chromatin Immunoprecipitation
Dot Blot
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| 免疫動物 |
Rabbit
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| 抗原部位 |
N-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 翻訳後修飾 |
メチル化
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| GENE ID |
121504
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| Accession No.(Gene/Protein) |
NP_001029249, P62805
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| Gene Symbol |
HIST4H4
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| 参考文献 |
[[Cosmo Reference Collection]][Pub Med ID]29123096
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| メーカー |
品番 |
包装 |
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RKL
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600-401-J01
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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