別品名 |
H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R2me2s/K4me2
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Immuno Fluorescence Chromatin Immunoprecipitation Dot Blot
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Caenorhabditis elegans
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翻訳後修飾 |
メチル化
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GENE ID |
126961
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Accession No.(Gene/Protein) |
NP_001005464, Q71DI3
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Gene Symbol |
HIST2H3C
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形状 |
滅菌済み液状品
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参考文献 |
Campsteijn C., OvrebO JI., Karlsen BO., Thompson EM. (2012). Expansion of cyclin D and CDK1 paralogs in Oikopleura dioica, a chordate employing diverse cell cycle variants. Mol. Biol. Evol. 2012 Feb. 29:487-502. Yue C., Soboloff J., Gamero AM. (2012). Control of type I interferon-induced cell seath by Orai1-mediated calcium entry in T cells. J Biol Chem. 2012 Jan. 287:3207-3216. Kung VL., Khare S., Stehlik C., Bacon EM., Hughes AJ., Hauser AR. (2012). An rhs gene of Pseudomonas aeruginosa encodes a virulence protein that activates the inflammasome. Proc Natl Acad Sci USA. 2012 Jan. 109:1275-1280. Sullivan CP., Berg EA., Elliot-Bryant R., Fishman JB., Mckee AC., Morin PJ., et al. (2011). Pyroglutamate-Αβ 3 and 11 colocalize in amyloid plaques in Alzheimer's disease cerebral cortex with pyroglutamate-Αβ 11 forming the central core. Neurosci Lett. 2011 Nov 14. 505(2):109-12. Daftarian P., Kaifer AE., Li W., Blomberg BB., Frasca D., Roth F., et al. (2011). Peptide-conjugated PAMAM dendrimer as a universal platform for antigen presenting cell targeting and effective DNA-based vaccinations. Cancer Res. 2011 Oct 10. 71(24):7452-62. Fishman J, Homon A, Lamsa E et al The Complete Chemical Synthesis of Histones H3 H4 Containing Epigenetic Modifications Their Use in Characterizing Arginine Methylated Histone Antibodies Poster presented at ASCB 21st Century Biochemicals, Marlborough, MA 01752, Novus Biologicals, LLC, 8100 Southpark Way, Littleton, CO 80120.
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Chromatin Immunoprecipitation of Rabbit Anti Histone H3 [Sym dimethyl Arg2, Dimethyl Lys4] Antibody. Chromatin from one million formaldehyde cross linked Hela cells was used with 2ug of Anti Histone H3 R2me2s K4me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real time PCR and SYBR green dye, then normalized to the non precipitated input chromatin, which is equal to one.
Immunofluorescence of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Dot Blot of Rabbit Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Lane 1: R2Me2. Lane 2: R2Me2/K4Me2. Lane 3: K4Me2. Load: 0.1, 1, 0.5, 5, and 10 picomoles of peptide. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight?488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Final Antibody reactivity to R2Me2/K4Me2 seen in the right blot.
Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Lane 1: C. elegans embryo cell lysate. Load: 30 ug per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 ug per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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Chromatin Immunoprecipitation of Rabbit Anti Histone H3 [Sym dimethyl Arg2, Dimethyl Lys4] Antibody. Chromatin from one million formaldehyde cross linked Hela cells was used with 2ug of Anti Histone H3 R2me2s K4me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real time PCR and SYBR green dye, then normalized to the non precipitated input chromatin, which is equal to one.
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メーカー |
品番 |
包装 |
RKL
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600-401-I85
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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