| 別品名 |
H3.3B, H3K27Me3, H3pS28K27Me3, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3 K27me3/pS28
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Immuno Fluorescence
Chromatin Immunoprecipitation
Dot Blot
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 交差種 |
Human
Mouse
Caenorhabditis elegans
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| 翻訳後修飾 |
リン酸化
メチル化
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| GENE ID |
126961
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| Accession No.(Gene/Protein) |
NP_001005464, Q71DI3
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| Gene Symbol |
HIST2H3C
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| 形状 |
滅菌済み液状品
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| 参考文献 |
[Pub Med ID]30909651
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Chromatin Immunoprecipitation Rabbit Anti Histone H3 [Trimethyl Lys27, p Ser28] Antibody. Chromatin from one million formaldehyde cross linked HeLa cells was used with 2ug of Anti Histone H3 K27me3pS28 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real time PCR and SYBR green dye, then normalized to the non precipitated input chromatin, which is equal to one.
Immunofluorescence of Rabbit Anti-Histone H3 [p Ser28, Trimethyl Lys27] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Ser28, Trimethyl Lys27] antibody at a 1:2000 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Ser28, Trimethyl Lys27] is nuclear and chromosomal. Staining: Histone H3 [p Ser28, Trimethyl Lys27] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Immunofluorescence of Rabbit Anti-Histone H3 [p Ser28, Trimethyl Lys27] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Ser28, Trimethyl Lys27] antibody at a 1:2000 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Ser28, Trimethyl Lys27] is nuclear and chromosomal. Staining: Histone H3 [p Ser28, Trimethyl Lys27] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Dot Blot of Rabbit Histone H3 [p Ser28, Trimethyl Lys27] Antibody. Lane 1: pS28/K27 unmodified. Lane 2: pS28 N-Term. Lane 3: p28 C-term. Lane 4: K27Me3. Lane 5: pS28/K27Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [p Ser28, Trimethyl Lys27] antibody at 1 ug/ml for 45 min at 4C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys27, p Ser28] Antibody. Lane 1: HeLa histone prep. Lane 2: NIH-3T3 histone prep. Lane 3: C. elegans embryo cell lysate. Load: 30 ug per lane. Primary antibody: Histone H3 [Trimethyl Lys27, p Ser28] at 1 ug/ml for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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Chromatin Immunoprecipitation Rabbit Anti Histone H3 [Trimethyl Lys27, p Ser28] Antibody. Chromatin from one million formaldehyde cross linked HeLa cells was used with 2ug of Anti Histone H3 K27me3pS28 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real time PCR and SYBR green dye, then normalized to the non precipitated input chromatin, which is equal to one.
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| メーカー |
品番 |
包装 |
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RKL
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600-401-I84
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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