別品名 |
H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9ac
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抗原部位 |
N-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Immuno Fluorescence Chromatin Immunoprecipitation Dot Blot
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Caenorhabditis elegans
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翻訳後修飾 |
アセチル化
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GENE ID |
126961
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Accession No.(Gene/Protein) |
NP_001005464, Q71DI3
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Gene Symbol |
HIST2H3C
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]29123096
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence of Rabbit Anti Histone H3 [ac Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [ac Lys9] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [ac Lys9] is nuclear and chromosomal. Staining: Histone H3 [ac Lys9] is expressed in green.
Dot Blot of Rabbit Histone H3 [ac Lys9] Antibody. Lane 1: K9. Lane 2: K9 Me1. Lane 3: K9 Me2. Lane 4: K9 Me3. Lane 5: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [ac Lys9] antibody at 1:1000 for 45 min at 4C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Anti-Histone H3 K9ac was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
Western Blot of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Lane 1: HeLa histone preps. Load: 30 ug per lane. Primary antibody: Histone H3 [ac Lys9] at 1:500 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 ug per lane. Primary antibody: Histone H3 [ac Lys9] at 1:500 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 ug per lane. Primary antibody: Histone H3 [ac Lys9] at 1:500 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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Immunofluorescence of Rabbit Anti Histone H3 [ac Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [ac Lys9] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [ac Lys9] is nuclear and chromosomal. Staining: Histone H3 [ac Lys9] is expressed in green.
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メーカー |
品番 |
包装 |
RKL
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600-401-I72
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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