Anti Histone H3 K9ac  

Immunofluorescence of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [ac Lys9] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [ac Lys9] is nuclear and chromosomal. Staining: Histone H3 [ac Lys9] is expressed in green.

Western Blot of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Lane 1: HeLa histone preps. Load: 30 μg per lane. Primary antibody: Histone H3 [ac Lys9] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800^TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Anti-Histone H3 K9ac was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

Dot Blot of Rabbit Histone H3 [ac Lys9] Antibody. Lane 1: K9. Lane 2: K9 Me1. Lane 3: K9 Me2. Lane 4: K9 Me3. Lane 5: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [ac Lys9] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight^TM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Western Blot of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 μg per lane. Primary antibody: Histone H3 [ac Lys9] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800^TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody: Histone H3 [ac Lys9] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800^TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

(d,？e)？293T cells were treated with AZD6738 or DMSO for 30？min and then fractionated. Nuclease-insoluble fraction was analyzed by mass spectrometry showing increase of the proteins involved in replication. Western blots confirm the MS results.？While ATR and RPA34 accumulate in the chromatin after treatments with DNA damaging agents, neither of these proteins were recruited to the chromatin in cells treated with ATRi alone in Fig.？1e. (p/n 600-401-I72, H3 acK9; 600-401-J01 H4, meK20) Fig 1. PMID:？29123096