別品名 |
H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R8me2s
|
抗原部位 |
N-terminus
|
種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Affinity Purified
|
適用 |
Western Blot Chromatin Immunoprecipitation Dot Blot
|
免疫動物 |
Rabbit
|
抗体クラス |
IgG
|
交差種 |
Human Caenorhabditis elegans
|
翻訳後修飾 |
メチル化
|
GENE ID |
126961
|
Accession No.(Gene/Protein) |
NP_001005464, Q71DI3
|
Gene Symbol |
HIST2H3C
|
形状 |
滅菌済み液状品
|
参考文献 |
Campsteijn C., OvrebO JI., Karlsen BO., Thompson EM. (2012). Expansion of cyclin D and CDK1 paralogs in Oikopleura dioica, a chordate employing diverse cell cycle variants. Mol. Biol. Evol. 2012 Feb. 29:487-502. Yue C., Soboloff J., Gamero AM. (2012). Control of type I interferon-induced cell seath by Orai1-mediated calcium entry in T cells. J Biol Chem. 2012 Jan. 287:3207-3216. Kung VL., Khare S., Stehlik C., Bacon EM., Hughes AJ., Hauser AR. (2012). An rhs gene of Pseudomonas aeruginosa encodes a virulence protein that activates the inflammasome. Proc Natl Acad Sci USA. 2012 Jan. 109:1275-1280. Sullivan CP., Berg EA., Elliot-Bryant R., Fishman JB., Mckee AC., Morin PJ., et al. (2011). Pyroglutamate-Αβ 3 and 11 colocalize in amyloid plaques in Alzheimer's disease cerebral cortex with pyroglutamate-Αβ 11 forming the central core. Neurosci Lett. 2011 Nov 14. 505(2):109-12. Daftarian P., Kaifer AE., Li W., Blomberg BB., Frasca D., Roth F., et al. (2011). Peptide-conjugated PAMAM dendrimer as a universal platform for antigen presenting cell targeting and effective DNA-based vaccinations. Cancer Res. 2011 Oct 10. 71(24):7452-62. Fishman J, Homon A, Lamsa E et al. The Complete Chemical Synthesis of Histones H3 and H4 Containing Epigenetic Modifications and Their Use in Characterizing Arginine Methylated Histone Antibodies. Poster presented at ASCB 21st Century Biochemicals, Marlborough, MA 01752, Novus Biologicals, LLC, 8100 Southpark Way, Littleton, CO 80120.
|
[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Chromatin Immunoprecipitation of Histone H3 [Sym dimethyl Arg8] Antibody. Chromatin from one million formaldehyde cross linked Hela cells was used with 2 ug of Anti Histone H3 R8 me2a was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT PCR and normalized to total input (input=1).
Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg8] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg8] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg8] Antibody. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 ug per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg8] at 2 ug/mL for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
|
|
Chromatin Immunoprecipitation of Histone H3 [Sym dimethyl Arg8] Antibody. Chromatin from one million formaldehyde cross linked Hela cells was used with 2 ug of Anti Histone H3 R8 me2a was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT PCR and normalized to total input (input=1).
|
|
|
メーカー |
品番 |
包装 |
RKL
|
600-401-I68
|
50 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
-20℃
|
|