別品名 |
H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R8me2a
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抗原部位 |
N-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Immunohistochemistry Immuno Fluorescence Chromatin Immunoprecipitation Dot Blot
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Caenorhabditis elegans
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翻訳後修飾 |
メチル化
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GENE ID |
126961
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Accession No.(Gene/Protein) |
NP_001005464, Q71DI3
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Gene Symbol |
HIST2H3C
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]27423858
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Chromatin Immunoprecipitation of Histone H3 [Asym dimethyl Arg8] Antibody. Chromatin from one million formaldehyde cross linked Hela cells was used with 2ug of Anti Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real time PCR and SYBR green dye, then normalized to the non precipitated input chromatin, which is equal to one.
Immunofluorescence of Rabbit Anti-Histone H3 [Asym-dimethyl Arg8] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Asym-dimethyl Arg8] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Asym-dimethyl Arg8] is nuclear and chromosomal. Staining: Histone H3 [Asym-dimethyl Arg8] is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Western Blot of Rabbit Anti-Histone H3 [Asym-dimethyl Arg8] Antibody. Lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate. Load: 30 ug per lane. Primary antibody: Histone H3 [Asym-dimethyl Arg8] at 1:500 for overnight at 4C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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Chromatin Immunoprecipitation of Histone H3 [Asym dimethyl Arg8] Antibody. Chromatin from one million formaldehyde cross linked Hela cells was used with 2ug of Anti Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real time PCR and SYBR green dye, then normalized to the non precipitated input chromatin, which is equal to one.
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メーカー |
品番 |
包装 |
RKL
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600-401-I67
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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