| 別品名 |
H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R8me2a
|
| 抗原部位 |
N-terminus
|
| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Immunohistochemistry
Immuno Fluorescence
Chromatin Immunoprecipitation
Dot Blot
|
| 免疫動物 |
Rabbit
|
| 抗体クラス |
IgG
|
| 交差種 |
Human
Mouse
Rat
Chicken
Caenorhabditis elegans
Drosophila
Plant
Xenopus
|
| 翻訳後修飾 |
メチル化
|
| GENE ID |
126961
|
| Accession No.(Gene/Protein) |
NP_001005464, Q71DI3
|
| Gene Symbol |
HIST2H3C
|
| 形状 |
滅菌済み液状品
|
| 参考文献 |
[Pub Med ID]27423858
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence of Rabbit Anti-Histone H3 [Asym-dimethyl Arg8] Antibody.
Tissue: HeLa cells.
Fixation: 0.5% PFA.
Antigen retrieval: Not required.
Primary antibody: Histone H3 [Asym-dimethyl Arg8] antibody at a 1:50 dilution for 1 h at RT.
Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT.
Localization: Histone H3 [Asym-dimethyl Arg8] is nuclear and chromosomal.
Staining: Histone H3 [Asym-dimethyl Arg8] is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Chromatin Immunoprecipitation of Histone H3 [Asym-dimethyl Arg8] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Western Blot of Rabbit Anti-Histone H3 [Asym-dimethyl Arg8] Antibody.
Lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate.
Load: 30 μg per lane.
Primary antibody: Histone H3 [Asym-dimethyl Arg8] at 1:500 for overnight at 4C.
Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block: 5% BLOTTO overnight at 4C.
Predicted/Observed size: ~15 kDa.
Other band(s): None.
|
|
|
|
Immunofluorescence of Rabbit Anti-Histone H3 [Asym-dimethyl Arg8] Antibody.
Tissue: HeLa cells.
Fixation: 0.5% PFA.
Antigen retrieval: Not required.
Primary antibody: Histone H3 [Asym-dimethyl Arg8] antibody at a 1:50 dilution for 1 h at RT.
Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT.
Localization: Histone H3 [Asym-dimethyl Arg8] is nuclear and chromosomal.
Staining: Histone H3 [Asym-dimethyl Arg8] is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
|
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
600-401-I67
|
50 UG
[0.84 mg/mL]
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
|
