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Immunofluorescence of Rabbit Anti-Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Monomethyl Lys4, p Thr6] is nuclear and chromosomal. Staining: Histone H3 [Monomethyl Lys4, p Thr6] is expressed in green and the nuclei are counterstained with DAPI (blue).
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Lane 1: C. elegans embryonic lysate. Load: 30 μg per lane. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Lane 1: HeLa histone preps. Load: 30 μg per lane. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Dot Blot of Rabbit Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Lane 1: unmodified. Lane 2: pT6. Lane 3: K4Me1 pT6. Lane 4: K4Me2 pT6. Lane 5: K4Me3 pT6. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight？488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
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Immunofluorescence of Rabbit Anti-Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Monomethyl Lys4, p Thr6] is nuclear and chromosomal. Staining: Histone H3 [Monomethyl Lys4, p Thr6] is expressed in green and the nuclei are counterstained with DAPI (blue).
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| 別品名 |
rabbit anti-Histone H3 monomethyl Lys4 pT6 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me1/pT6
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| 交差種 |
Human
Caenorhabditis elegans
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| 適用 |
Western Blot
Immuno Fluorescence
Dot Blot
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| 免疫動物 |
Rabbit
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| 抗原部位 |
N-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 翻訳後修飾 |
リン酸化
メチル化
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| GENE ID |
126961
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| Accession No.(Gene/Protein) |
NP_001005464, Q71DI3
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| Gene Symbol |
HIST2H3C
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| 参考文献 |
[Pub Med ID]30909651
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-I61
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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