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Immunofluorescence Microscopy of Rabbit Anti-Histone H3 [p Thr3, Dimethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Thr3, Dimethyl Lys4] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Thr3, Monomethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [p Thr3, Dimethyl Lys4] is expressed in green while the nuclei and alpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
Western Blot of Rabbit Anti-Histone H3 [p Thr3, Dimethyl Lys4] Antibody. Lane 1: NIH-3T3 histone prep lysate. Load: 30 μg per lane. Primary antibody: Histone H3 [p Thr3, Dimethyl Lys4] at 1:50 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [p Thr3, Dimethyl Lys4] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Hisone H3 K4 Me2 pT3 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Dot Blot of Rabbit Histone H3 [p Thr3, Dimethyl Lys4] Antibody. Antigen: Methylated forms of the immunizing peptide. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [p Thr3, Dimethyl Lys4] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight？488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
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Immunofluorescence Microscopy of Rabbit Anti-Histone H3 [p Thr3, Dimethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Thr3, Dimethyl Lys4] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Thr3, Monomethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [p Thr3, Dimethyl Lys4] is expressed in green while the nuclei and alpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
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| 別品名 |
rabbit anti-Histone H3 dimethyl Lys4 pT3 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87782, MGC87783, H3K4me2/pT3
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| 交差種 |
Human
Mouse
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| 適用 |
Western Blot
Immuno Fluorescence
Chromatin Immunoprecipitation
Dot Blot
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| 免疫動物 |
Rabbit
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| 抗原部位 |
N-terminus
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 翻訳後修飾 |
リン酸化
メチル化
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| GENE ID |
126961
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| Accession No.(Gene/Protein) |
NP_001005464, Q71DI3
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| Gene Symbol |
HIST2H3C
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| 参考文献 |
Campsteijn C., OvrebO JI., Karlsen BO., Thompson EM. (2012). Expansion of cyclin D and CDK1 paralogs in Oikopleura dioica, a chordate employing diverse cell cycle variants. Mol. Biol. Evol. 2012 Feb. 29:487-502. Yue C., Soboloff J., Gamero AM. (2012). Control of type I interferon-induced cell seath by Orai1-mediated calcium entry in T cells. J Biol Chem. 2012 Jan. 287:3207-3216. Kung VL., Khare S., Stehlik C., Bacon EM., Hughes AJ., Hauser AR. (2012). An rhs gene of Pseudomonas aeruginosa encodes a virulence protein that activates the inflammasome. Proc Natl Acad Sci USA. 2012 Jan. 109:1275-1280. Sullivan CP., Berg EA., Elliot-Bryant R., Fishman JB., Mckee AC., Morin PJ., et al. (2011). Pyroglutamate-Αβ 3 and 11 colocalize in amyloid plaques in Alzheimer's disease cerebral cortex with pyroglutamate-Αβ 11 forming the central core. Neurosci Lett. 2011 Nov 14. 505(2):109-12. Daftarian P., Kaifer AE., Li W., Blomberg BB., Frasca D., Roth F., et al. (2011). Peptide-conjugated PAMAM dendrimer as a universal platform for antigen presenting cell targeting and effective DNA-based vaccinations. Cancer Res. 2011 Oct 10. 71(24):7452-62.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-I55
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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