| 別品名 |
HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
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| 種由来 |
Mouse
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| 適用 |
Western Blot
Immunoprecipitation
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| 免疫動物 |
Rat
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| 抗体クラス |
IgG
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| クローン |
eB144
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| 交差種 |
Mouse
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| 形状 |
滅菌済み液状品
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| 構成内容 |
ミニゲルで約20ブロット分の試薬が含まれます。
●Mouse IgG TrueBlot ULTRA: 50 μl
●TrueBlot Enhancer Solution: 25 ml
●TrueBlot Blocker: 10 g
●TrueBlot Assay Buffer: 30 ml. 20X
●TrueBlot Substrate A: 12.5 ml
●TrueBlot Substrate B: 12.5 ml
●ウエスタンブロットインキュベーショントレイ
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| 必須品 |
TrueBlot Anti-Mouse Ig IP Beads 品番00-8811-25と組み合わせてお使いください｡
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| 参考文献 |
[Pub Med ID]32359397, 36898832
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※サムネイル画像をクリックすると拡大画像が表示されます。
Mouse TrueBlotR IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Mouse TrueBlotR ULTRA: Anti-Mouse Ig HRP (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlotR ULTRA: Anti-Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
Rockland produces kits to meet the requirements of researchers for highly sensitive antibody based assays. Western Blot Kit Components: 1. IgG TrueBlot: 50 μL. 2. TrueBlot Enhancer Solution: 25 mL; 3. TrueBlot Blocker: 10 g; 4. TrueBlot Assay Buffer: 30 mL, 20X; 5. TrueBlot Substrate A: 12.5 mL; 6. TrueBlot Substrate B: 12.5 mL; 7. Western Blot Incubation Tray. 8. Anti-Alpha-Tubulin (MOUSE) Monoclonal Antibody - 200-301-880S.
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Mouse TrueBlotR IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Mouse TrueBlotR ULTRA: Anti-Mouse Ig HRP (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlotR ULTRA: Anti-Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
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| メーカー |
品番 |
包装 |
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RKL
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88-8887-31
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1 KIT
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃と−２０℃
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