TrueBlot(R) Western Blot Kit  

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別品名 HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
種由来 Goat
適用 Western Blot
免疫動物 Mouse
抗体クラス IgG
クローン eB270
交差種 Goat
形状 滅菌済み液状品
構成内容 ミニゲルで約20ブロット分の試薬が含まれます。
●Goat IgG TrueBlot: 50 μl
●TrueBlot Enhancer Solution: 25 ml
●TrueBlot Blocker: 10 g
●TrueBlot Assay Buffer: 30 ml. 20X
●TrueBlot Substrate A: 12.5 ml
●TrueBlot Substrate B: 12.5 ml
必須品 TrueBlot Anti-Goat Ig IP Beads 品番00-8844-25と組み合わせてお使いください。
参考文献 Kong, D., L. Xu, Y. Yu, W. Zhu, D.W. Andrews, Y. Yoon, and T.H. Kuo. 2005. Regulation of Ca2+-induced permeability transition by BCL-2 is antagonized by Drp1 and hFis1. Molecular and Cellular Biochemistry. 272: 187-199. (Rabbit IgG TrueBlot, PubMed) DiPerna, G., J. Stack, A.G. Bowie, A. Boyd, G. Kotwal, Z. Zhang, S. Arvikar, E. Latz, K.A. Fitzgerald, and W.L. Marshall. 2004. Poxvirus protein N1L targets the I-kappaB Kinase complex, inhibits signaling to NF-kappaB by the Tumor Necrosis Factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by Toll-like Receptors. J. Biol. Chem. 279: 36570-36578. (Rabbit IgG TrueBlot, PubMed) Zhang, X., Y. Ozawa, H. Lee, Y. Wen, T. Tan, B. Wadzinski, and E. Seto. 2005. Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4. Genes & Development. 19: 827-839. (Rabbit IgG TrueBlot, PubMed) Lehtonen, S., E. Lehtonen, K. Kudlicka, H. Holthofer, and M.G. Farquhar. 2004. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am J Pathol. 165:923-936. (Rabbit IgG TrueBlot, PubMed) Tyagi A, Agarwal C, Harrison G, Glode LM, Agarwal R. 2004. Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages. Carcinogenesis. 25: 1711-20. (Mouse IgG TrueBlot, PubMed)


Goat TrueBlotR IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with goat anti human Stat1 and immunoprecipitated using Protein G. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti Stat1 using Goat TrueBlotR: Anti Goat IgG HRP (lane 1) and conventional HRP conjugated anti goat polyclonal antibody (lane 2).

メーカー 品番 包装
RKL 88-8884-31 1 KIT
希望販売価格 ¥82,000


当社在庫 なし
納期目安 約10日
保存温度 4℃と−20℃




メーカー名 Rockland Immunochemicals, Inc.
略号 RKL