| 別品名 |
HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
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| 種由来 |
Goat
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| 適用 |
Western Blot
Immunoprecipitation
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| 免疫動物 |
Mouse
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| 抗体クラス |
IgG
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| クローン |
eB270
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| 交差種 |
Goat
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| 形状 |
滅菌済み液状品
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| 構成内容 |
ミニゲルで約20ブロット分の試薬が含まれます。
●Goat IgG TrueBlot: 50 μl
●TrueBlot Enhancer Solution: 25 ml
●TrueBlot Blocker: 10 g
●TrueBlot Assay Buffer: 30 ml. 20X
●TrueBlot Substrate A: 12.5 ml
●TrueBlot Substrate B: 12.5 ml
●ウエスタンブロットインキュベーショントレイ
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| 必須品 |
TrueBlot Anti-Goat Ig IP Beads 品番00-8844-25と組み合わせてお使いください｡
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| 参考文献 |
Kong, D., L. Xu, Y. Yu, W. Zhu, D.W. Andrews, Y. Yoon, and T.H. Kuo. 2005. Regulation of Ca2+-induced permeability transition by BCL-2 is antagonized by Drp1 and hFis1. Molecular and Cellular Biochemistry. 272: 187-199. (Rabbit IgG TrueBlot, PubMed) DiPerna, G., J. Stack, A.G. Bowie, A. Boyd, G. Kotwal, Z. Zhang, S. Arvikar, E. Latz, K.A. Fitzgerald, and W.L. Marshall. 2004. Poxvirus protein N1L targets the I-kappaB Kinase complex, inhibits signaling to NF-kappaB by the Tumor Necrosis Factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by Toll-like Receptors. J. Biol. Chem. 279: 36570-36578. (Rabbit IgG TrueBlot, PubMed) Zhang, X., Y. Ozawa, H. Lee, Y. Wen, T. Tan, B. Wadzinski, and E. Seto. 2005. Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4. Genes & Development. 19: 827-839. (Rabbit IgG TrueBlot, PubMed) Lehtonen, S., E. Lehtonen, K. Kudlicka, H. Holthofer, and M.G. Farquhar. 2004. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am J Pathol. 165:923-936. (Rabbit IgG TrueBlot, PubMed) Tyagi A, Agarwal C, Harrison G, Glode LM, Agarwal R. 2004. Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages. Carcinogenesis. 25: 1711-20. (Mouse IgG TrueBlot, PubMed)
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※サムネイル画像をクリックすると拡大画像が表示されます。
Goat TrueBlotR IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with goat anti human Stat1 and immunoprecipitated using Protein G. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti Stat1 using Goat TrueBlotR: Anti Goat IgG HRP (lane 1) and conventional HRP conjugated anti goat polyclonal antibody (lane 2).
Rockland produces kits to meet the requirements of researchers for highly sensitive antibody based assays. Western Blot Kit Components: 1. IgG TrueBlot: 50 μL. 2. TrueBlot Enhancer Solution: 25 mL; 3. TrueBlot Blocker: 10 g; 4. TrueBlot Assay Buffer: 30 mL, 20X; 5. TrueBlot Substrate A: 12.5 mL; 6. TrueBlot Substrate B: 12.5 mL; 7. Western Blot Incubation Tray. 8. Anti-Alpha-Tubulin (MOUSE) Monoclonal Antibody - 200-301-880S.
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Goat TrueBlotR IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with goat anti human Stat1 and immunoprecipitated using Protein G. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti Stat1 using Goat TrueBlotR: Anti Goat IgG HRP (lane 1) and conventional HRP conjugated anti goat polyclonal antibody (lane 2).
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| メーカー |
品番 |
包装 |
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RKL
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88-8884-31
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1 KIT
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃と−２０℃
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