| 別品名 |
Anti-Mouse immunoglobulin Gamma, Agarose-conjugated IgG, Anti-Ms IgG, Anti-Mouse IgG, TrueBlot, TrueBlot for immunoprecipitation, IP beads for TrueBlot, HRP, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for western blotting
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| 種由来 |
Mouse
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| 適用 |
Western Blot
Immunoprecipitation
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| 免疫動物 |
Rat
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| 抗体クラス |
IgG
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| クローン |
eB144
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| 交差種 |
Mouse
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| 形状 |
滅菌済み液状品
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| 構成内容 |
ミニゲルで約20ブロット分の試薬が含まれます。
●Mouse IgG TrueBlotR （HRP標識Anti Mouse IgG試薬）
●Anti-Mouse Ig IP ビーズ: 2.5 ml （結合能： 0.4 mg Ig/ml beads）
●ウエスタンブロットインキュベーショントレイ
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| 参考文献 |
[Pub Med ID]27209022, 27584860, 30415951
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※サムネイル画像をクリックすると拡大画像が表示されます。
Mouse TrueBlotR IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Mouse TrueBlotR ULTRA: Anti-Mouse Ig HRP (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlotR ULTRA: Anti-Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
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Mouse TrueBlotR IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Mouse TrueBlotR ULTRA: Anti-Mouse Ig HRP (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlotR ULTRA: Anti-Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
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| メーカー |
品番 |
包装 |
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RKL
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88-7788-31
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1 SET
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃と−２０℃
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