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ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG2a Pre-adsorbed secondary antibody. Lane 1: Mouse IgG2a. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 647N goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a. Other band(s): none.
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ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
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| 別品名 |
goat anti-MOUSE IgG2a Antibody ATTO647N Conjugation, goat anti-Mouse IgG2a (gamma 2a) ATTO 647N Conjugated Antibody
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| 交差種 |
Mouse
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| 非交差(吸収処理)種 |
Human Bovine Rabbit
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| 適用 |
Western Blot
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| 免疫動物 |
Goat
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| 標識物 |
ATTO 647N
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]33380421
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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610-156-041
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500 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-20℃
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