別品名 |
IgG2a antibody, mouse IgG2a, anti-subclass secondary antibody, AT647N, ATTO 647N, ATTO-TEC 647N
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種由来 |
Mouse
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標識物 |
ATTO 647N
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精製度 |
Affinity Purified
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適用 |
Western Blot
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免疫動物 |
Goat
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交差種 |
Mouse
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非交差(吸収処理)種 |
Human Bovine Rabbit
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形状 |
凍結乾燥品
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参考文献 |
[Pub Med ID]33380421, 34995113, 35177616
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2 male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha actin was stained with a mouse primary antibody and ATTO 488 anti mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti cytokeratin and ATTO 647N anti rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti Vimentin ATTO 532 (green), anti E Cadherin ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti Aquaporin 3 ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. J'rg Reichwein, ATTO TEC GmbH
Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG2a Pre-adsorbed secondary antibody. Lane 1: Mouse IgG2a. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 647N goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a. Other band(s): none.
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ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2 male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha actin was stained with a mouse primary antibody and ATTO 488 anti mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti cytokeratin and ATTO 647N anti rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti Vimentin ATTO 532 (green), anti E Cadherin ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti Aquaporin 3 ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. J'rg Reichwein, ATTO TEC GmbH
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メーカー |
品番 |
包装 |
RKL
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610-156-041
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500 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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