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ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 μg/mL final concentration of anti-a-tubulin and detected with 0.2 μg/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] (610-151-121) secondary antibody (colored RED). Also shown in this 2-color STED image is Rockland’s Anti-HDAC-1 [RABBIT] (p/n 600-401-879) detected with DyLight?488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Image courtesy of Myriam Gastard, Leica Microsystems, USA.
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ATTO R dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jorg Reichwein, ATTO-TEC GmbH
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| 別品名 |
Goat anti-mouse IgG2a antibody ATTO425 conjugation, goat anti-mouse IgG2a (gamma 2a) ATTO 425 conjugated antibody
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| 交差種 |
Mouse
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| 非交差(吸収処理)種 |
Human Bovine Rabbit
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| 適用 |
Dot Blot
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| 免疫動物 |
Goat
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| 標識物 |
ATTO 425
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]29686261
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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610-151-041
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500 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-20℃
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