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Immunohistochemistry of Rabbit anti-Amyloid Fibrils antibody. Tissue: (A) hippocampus, (B) subiculum, (C) temporal cortex, and (D) dense and fine fibrillar material. Fixation: N/A. Primary Antibody: Amyloid Fibrils antibody at 1ug/ml for 1h at RT. Secondary antibody: Peroxidase rabbit secondary at 1:10,000 for 45 min at RT. Localization: Membrane. Staining: Amyloid Fibrils as precipitated brown signal with hematoxylin purple nuclear counterstain.
Dot Blot of Rabbit anti-Amyloid Fibrils antibody. Antigen: Beta Amyloid HEPES-NaCl aggregation. Primary antibody: Amyloid Fibrils antibody at 1:500 (lane 1) and 1:5000 (lane 2) for 45 min at 4°C. Secondary antibody: Peroxidase rabbit Secondary antibody at 1:10,000 time lapse. Block: 5% BLOTTO overnight at 4°C.
Dot Blot of Rabbit Amyloid Fibrils (OC) antibody. Antigen: Aβ42 and polyQ36 prefibrillar oligomers and fibrils. Load: 2ug per dot. Primary antibody: Top row: Amyloid Oligomers (A11) or bottom row: Amyloid Fibrils (OC) at 1:400 for 45 min at 4°C. Secondary Antibody: Goat anti-rabbit IgG HRP at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight at 4°C. Amyloid Fibrils (OC) reacts to Aβ42 fibrils and polyQ36 fibrils only.
Western Blot of rabbit Anti-Amyloid Fibrils Antibody. Lane 1 and 3: (F) Fibrils. Lane 2 and 4: (O) prefibrillar oligomers. Load: 10 ug per lane. Primary antibody: Anti-Amyloid Fibrils or Anti-Oligomers at 1:1000 for overnight at 4°C . Secondary antibody: Goat anti-rabbit IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4°C. Predicted/Observed size: 18kDa on left blot (OC) in lane one.
Immunohistochemistry of Rabbit anti-Amyloid Fibrils antibody. Tissue: Human AD Brain. Fixation: N/A. Primary Antibody: (Left) Amyloid Fibril antibody, (Right) Monoclonal 6E10 at 1ug/ml for 1h at RT. Secondary antibody: Peroxidase rabbit secondary at 1:10,000 for 45 min at RT. Localization: Membrane. Staining: (Left) Amyloid Fibrils as precipiated brown signal with no cross reactivity with Amyloid Precursor Protein (APP), (right) considerable cross reactivity.
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Immunohistochemistry of Rabbit anti-Amyloid Fibrils antibody. Tissue: (A) hippocampus, (B) subiculum, (C) temporal cortex, and (D) dense and fine fibrillar material. Fixation: N/A. Primary Antibody: Amyloid Fibrils antibody at 1ug/ml for 1h at RT. Secondary antibody: Peroxidase rabbit secondary at 1:10,000 for 45 min at RT. Localization: Membrane. Staining: Amyloid Fibrils as precipitated brown signal with hematoxylin purple nuclear counterstain.
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| 別品名 |
Amyloid OC, Fibrils, Amyloid Oligomer αβ, A11, Amyloid beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, APP, A4, AD1
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| 交差種 |
Human
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| 適用 |
Western Blot
Immunohistochemistry
Immuno Fluorescence
Immunoprecipitation
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| Accession No.(Gene/Protein) |
P05067
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| Gene Symbol |
APP
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| 参考文献 |
[Pub Med ID]31693761
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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200-401-E87
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100 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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