Anti Amyloid Fibrils (OC)  

Immunohistochemistry of Rabbit anti-Amyloid Fibrils antibody. Tissue: (A) hippocampus, (B) subiculum, (C) temporal cortex, and (D) dense and fine fibrillar material. Fixation: N/A. Primary Antibody: Amyloid Fibrils antibody at 1ug/ml for 1h at RT. Secondary antibody: Peroxidase rabbit secondary at 1:10,000 for 45 min at RT. Localization: Membrane. Staining: Amyloid Fibrils as precipitated brown signal with hematoxylin purple nuclear counterstain.

Dot Blot of Rabbit anti-Amyloid Fibrils antibody. Antigen: Beta Amyloid HEPES-NaCl aggregation. Primary antibody: Amyloid Fibrils antibody at 1:500 (lane 1) and 1:5000 (lane 2) for 45 min at 4°C. Secondary antibody: Peroxidase rabbit Secondary antibody at 1:10,000 time lapse. Block: 5% BLOTTO overnight at 4°C.

Dot Blot of Rabbit Amyloid Fibrils (OC) antibody. Antigen: Aβ42 and polyQ36 prefibrillar oligomers and fibrils. Load: 2ug per dot. Primary antibody: Top row: Amyloid Oligomers (A11) or bottom row: Amyloid Fibrils (OC) at 1:400 for 45 min at 4°C. Secondary Antibody: Goat anti-rabbit IgG HRP at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight at 4°C. Amyloid Fibrils (OC) reacts to Aβ42 fibrils and polyQ36 fibrils only.

Western Blot of rabbit Anti-Amyloid Fibrils Antibody. Lane 1 and 3: (F) Fibrils. Lane 2 and 4: (O) prefibrillar oligomers. Load: 10 ug per lane. Primary antibody: Anti-Amyloid Fibrils or Anti-Oligomers at 1:1000 for overnight at 4°C . Secondary antibody: Goat anti-rabbit IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4°C. Predicted/Observed size: 18kDa on left blot (OC) in lane one.

Immunohistochemistry of Rabbit anti-Amyloid Fibrils antibody. Tissue: Human AD Brain. Fixation: N/A. Primary Antibody: (Left) Amyloid Fibril antibody, (Right) Monoclonal 6E10 at 1ug/ml for 1h at RT. Secondary antibody: Peroxidase rabbit secondary at 1:10,000 for 45 min at RT. Localization: Membrane. Staining: (Left) Amyloid Fibrils as precipiated brown signal with no cross reactivity with Amyloid Precursor Protein (APP), (right) considerable cross reactivity.

(a) Structural characterization of Aβ40 monomers; (b) oligomers; and (c) fibrils by conventional TEM. TEM images were taken from Aβ40 samples with (a) no incubation; (b) incubation at 37 °C for 6 days; and (c) incubation at 37 °C for 10 days; (d) The aggregation characteristics of Aβ40 solutions with different DMSO concentrations. To estimate the extent of Aβ40 aggregation, we employed rGO sensors with OC antibodies, which specifically interacted with the fibrils. The ΔR2？values of the rGO sensors were measured when Aβ40 solutions with different incubation times were added at each DMSO concentration (n？= 12); (e) Performance test of the rGO sensors with respect to the concentration of each conformation of Aβ40 (n？= 7). Three different types of antibodies as receptors for capturing monomers, oligomers, and fibrils of Aβ40, including monoclonal 6E10 antibodies specific for Aβ sequence 1？16, polyclonal A11 antibodies (p/n 200-401-E88) for Aβ40 oligomers, and polyclonal OC antibodies (p/n 200-401-E87) for Aβ40 fibrils. Fig 3. PMID:？29843431

(a) Atomic force microscopy (AFM) images of Aβ40 samples incubated for 10 days without or (b) with 4-(2-hydroxyethyl)-1-piperazinepropanesulphonic acid (EPPS) treatment. The Aβ40 solution incubated for 10 days was treated with EPPS for 24 h and then analyzed by AFM. (c) The ΔR2？values of the sensors with respect to EPPS treatment time (no treatment and 24 h); (d) Relative ratios of ΔR2？values for monomers, oligomers, and fibrils. Three different types of antibodies as receptors for capturing monomers, oligomers, and fibrils of Aβ40, including monoclonal 6E10 antibodies specific for Aβ sequence 1？16, polyclonal A11 antibodies (p/n 200-401-E88) for Aβ40 oligomers, and polyclonal OC antibodies (p/n 200-401-E87) for Aβ40 fibrils. Fig 5. PMID:？29843431

Effect of 16hr-incubation at 37 ？ C on the conformation of Aβ. 1μM Aβ42 was incubated in cell culture media, with or without 50μM FAC, for 16 hrs. Before and after the incubation, fractions of each solution were used to assess changes in Aβ conformation by dot blot analyses. Primary antibodies A11 (1:1000 dilution), OC (1:1000 dilution) and 4G8 (1:1000 dilution) were used to detect, oligomeric, fibrillar and total Aβ respectively. Two-tailed student’s t-test was used to determine the significance of the data. ns denotes no significant difference. Data are represented as mean ± SD from 4 independent experiments (n = 1 incubation per experiment). Polyclonal A11 antibodies (p/n 200-401-E88) for Aβ40 oligomers, and polyclonal OC antibodies (p/n 200-401-E87) for Aβ40 fibrils. Figure S2. PMID:？31693761