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Western Blot of Rabbit anti- CaM Kinase II Thr286 Antibody. Lane 1: rat brain lysate. Lane 2: rat brain lysate incubated in λ-Ptase (1200 units for 30 min). Load: 10 μg per lane. Primary antibody: CaM Kinase II Thr286 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50kDa α- and the ~60kDa for β-CaM Kinase II phosphorylated at Thr286. Other band(s): none.
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Western Blot of Rabbit anti- CaM Kinase II Thr286 Antibody. Lane 1: rat brain lysate. Lane 2: rat brain lysate incubated in λ-Ptase (1200 units for 30 min). Load: 10 μg per lane. Primary antibody: CaM Kinase II Thr286 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800？ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50kDa α- and the ~60kDa for β-CaM Kinase II phosphorylated at Thr286. Other band(s): none.
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| 別品名 |
Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, Camk2a protein
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| 交差種 |
Mouse
Rat
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 翻訳後修飾 |
リン酸化
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| GENE ID |
25400
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| Accession No.(Gene/Protein) |
NP_037052.1, P11275
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| Gene Symbol |
Camk2A/B
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| 参考文献 |
Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP and Silva AJ (2002). Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning. Neuron 36:493-505. Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G and Fetcho JR (2003). Translocation of CaM kinase II to synaptic sites in vivo. Nature Neurosci 6:217-218. Hudmon A and Schulman H (2002). Neuronal Ca2+/calmodulin-dependent protein kinase II: The role of structure and autoregulation in cellular function. Annu Rev Biochem 71:473-510. Kennedy MB (1998). Signal transduction molecules at the glutamatergic postsynaptic membrane. Brain Res Rev 26:243-257. Meng FJ, Guo J, Zhang QG, Song B and Zhang GY (2003). Autophosphorylated calcium/calmodulin-dependent protein kinase IIa (CaMKIIa) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats. Brain Res 967:161-169. Picciotto MR, Czernik AJ and Nairn AC (1993). Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site. J Biol Chem 268:26512-26521. Sun X, Milovanovic M, Zhao Y and Wolf ME. (2008). Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons. J Neurosci. Apr 16;28(16):4216-30. Vest R, O'Leary H, Coultrap S, Kindy M and Ulrich Bayer K (2010) Effective Post-insult Neuroprotection by a Novel Ca2+/ Calmodulin-dependent Protein Kinase II (CaMKII) InhibitorJ. Biol. Chem., 285: 20675 - 20682. Davies K, Goebel-Goody S, Coultrap S and Browning M (2008). Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization J. Biol. Chem., 283: 33138 - 33146. Sun X, Milovanovic M, Zhao Y and Wolf M (2008). Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 - 4230. Vest R, Davies K, O'Leary H, Port J and Ulrich Bayer K (2007). Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024 - 5033. Townsend M, Mehta T and Selkoe D (2007). Soluble A Inhibits Specific Signal Transduction Cascades Common to the Insulin Receptor Pathway. J. Biol. Chem., 282: 33305 - 33312.
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| メーカー |
品番 |
包装 |
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RKL
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612-401-C71
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100 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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