Anti IgG (H&L)

Western Blot of Unconjugated Anti Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603 701 C37). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003 0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003 0103). Lane 3: Chicken IgG Fab Fragment (p/n 003 0105). Lane 4: Chicken IgM Whole Molecule (p/n 003 0107). Lane 5: Chicken Serum (p/n D302 05). All samples were reduced. Load: 50 ng per lane. Block: MB 070 for 30 min at RT. Primary Antibody: Anti Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603 701 C37) 1:3,000 for 60 min at RT. Secondary antibody: Anti Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616 1302) 1:40,000 in MB 070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.