Anti IgG (H&L)

DyLightTM dyes can be used for multi color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLightTM dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti histone detection using a DyLightTM 488 conjugate (green). Anti Tubulin was detected using a DyLightTM 549 conjugate (red). Nuclei were counter stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.

DyLightTM 488 Fluorescence Spectra.

Properties of DyLightTM Fluorescent Dyes.