Anti GLI2  

Western blot using Rockland's affinity purified anti-Gli-2 antibody shows detection of Gli-2 protein. Lane 1: rat testes (p/n W12-000-GZ3) and Lane 2: human HEK293 (p/n W09-000-365) whole cell lysates (arrowhead).？ See Ruppert et al for testing conditions.？ Each lane contains approximately 35μg of lysate.？ Primary antibody was used at a 1:400 dilution in 5% BLOTTO (p/n B501-0500) in PBS overnight at 4°C.？ The membrane was washed and reacted with a 1:10,000 dilution of IRDyeR 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green).？？ Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).？？ IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.？ Other detection systems will yield similar results.