Anti Collagen Type I

Immunohistochemistry results of Rabbit Anti Collagen Type I Antibody. Tissue: human stomach mucosa (TMA). Fixation: FFPE. Antigen Retrieval: HIER using Tris EDTA citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase Blocking Solution for 10 min. Primary Antibody: Anti Collagen Type I (p/n 600 401 103 0.1) at 1:15 for 1 hr at 37 C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec.Substrate: DAB Chromogen, Rabbit/Mouse. Staining/Results: basement membranes and blood vessels.

Immunohistochemistry results of Rabbit Anti-Collagen Type I Antibody. Tissue: smooth muscle cells of human stomach wall. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type I (p/n 600-401-103-0.1) at 1:15 for 1 hr at 37 C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec.Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: smooth muscle cells surrounded by collagen fibers.

Immunohistochemistry results of Rabbit Anti-Collagen Type I Antibody. Tissue: human tubuli and blood vessels. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type I (p/n 600-401-103-0.1) at 1:15 for 1 hr at 37 C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec.Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: Intense collagen I staining of fibres surrounding tubuli and around blood vessels.

Immunohistochemistry results of Rabbit Anti-Collagen Type I Antibody. Tissue: human stroma of a colorectal adenocarcinoma. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type I (p/n 600-401-103-0.1) at 1:15 for 1 hr at 37 C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec.Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: Cancer cells are collagen I negative.

Human AF matrix biochemical characterization with herniation progression. A) Histological/IHC staining for a？d: Alcian Blue/Picro-Sirius Red, scale bar: 100？μm; e？h: Collagen I, scale bar: 100？μm; i？l: Collagen II, scale bar: 100？μm; m？p: Fibronectin, scale bar: 100？μm. B) Quantification of each staining per herniation type. Data presented using dot plots, with median and interquartile range. Kruskal-Wallis test followed by corrected Dunn’s were performed. *p < 0.05; **p < 0.01. C) Multivariate analysis of interaction of hernia containment level with age for each staining Figure provided by CiteAb. Source: Arthritis Res Ther, PMID: 35039075.

Compound 2 suppressed the collagenolytic effect of ColQ1 ex vivo in pig skin tissue. a) Dose-dependent effect of compound 2 quantified by Hyp release assay. b) Confocal SHG images showed an improved COL signal with 5 10？6 m of compound 2 (tissue challenged with 300 ColQ1) compared with 300 ColQ1 without inhibitor. c) Immunostaining of fibrillar COLs of the non-treated pig skin and treated with ColQ1 with or without compound 2. Statistical analysis was performed with one-way ANOVA and statistical significance was analyzed by Tukey test. Significance was calculated by comparing non-treated versus treated tissue with compound 2 (mean +- SD, *** p 0.001, ** p 0.01). Hyp: hydroxyproline, COL: collagen, SHG: second harmonic generation. Scale bar: 100 ？om for SHG images and 100 ？om for the immunostained images. Figure provided by CiteAb. Source: Adv Ther (Weinh), PMID: 35310821.

ACLP signaling represses adipogenesis. A, Scheme of 10T1/2 adipogenesis and rACLP treatment. B, 10T1/2 fibroblasts were treated with 30 nM rACLP on days -2, -1, 0, +2 and +4 and induced to undergo adipogenesis with DMII on day 0. Protein was harvested on day +6 and analyzed by SDS-PAGE and Western blot with antibodies against ACLP, collagen, SMA, AdipoQ, FABP4, PPAR and cyclophilin-a (CypA). PPAR analysis was performed in separate experiments. Protein expression was quantified by densitometry normalized to CypA expression and relatively compared to control cells. *, p < 0.05 versus control, one sample t-test for all values. C, 10T1/2 fibroblasts were treated with 30 nM rACLP on days -2, -1, 0, +2 and +4 and induced to undergo adipogenesis with DMII on day 0. On day +6 cells were fixed, stained, imaged and quantified with Oil Red O dye (n = 3). Data were normalized relative to untreated controls. *, p < 0.05 versus paired control, one sample t-test. Data presented are expressed as mean +- SD. The scale bar represents 2 mm. Figure provided by CiteAb. Source: PLoS One, PMID: 29799877.

Recombinant ACLP inhibits adipogenesis and enhances myofibroblast differentiation in human adipose stromal cells. A, Scheme of time course for adipogenesis of hASC. B, hASC derived from subcutaneous adipose depots were induced to undergo adipogenesis with CDM on day 0. Protein was harvested on days 0, +1, +2, +8 and +15. Protein expression was analyzed using SDS-PAGE and Western blot with antibodies against ACLP, SMA, FABP4 and cyclophilin-A. C, Omental hASC were induced to undergo adipogenesis with CDM on day 0. Protein was harvested on days 0, +1, +2, +8 and +15. Protein expression was analyzed using SDS-PAGE and Western blot with antibodies against ACLP, -SMA, FABP4 and cyclophilin-A. D, Scheme of adipogenesis for hASC and rACLP treatment. E, Subcutaneous hASC were treated with 30 nM rACLP on days -2, -1 and 0 and induced to undergo adipogenesis with CDM on day 0 and +4. Protein was harvested on day +15 and analyzed by SDS-PAGE and Western blot with antibodies against collagen, -SMA, perilipin, FABP4 and CypA (n = 3). F, Subcutaneous hASC were treated with 30 nM rACLP on days -2, -1, and 0 and induced to undergo adipogenesis with CDM on day 0 and +4. On day +15 cells were fixed, stained, imaged and quantified with Oil Red O dye (n = 5). Data were normalized relative to control. * p < 0.05 versus control. Data presented are expressed as mean +- SD. The scale bar represents 2 mm. Figure provided by CiteAb. Source: PLoS One, PMID: 29799877.

Immunofluorescence staining of extracellular and intracellular type I collagen. (a) Immunofluorescence staining of type I collagen secreted from MEF clones was performed with an anti-type I collagen antibody without cell permeabilization. Scale bars: 100 μm. (b,c) Immunofluorescence staining of permeabilized MEF clones was performed with anti-type I collagen (green) and anti-KDEL antibodies (red) (b) or anti-type I collagen (green) and anti-GM130 antibodies (red). (c) Scale bars: 10 μm. Figure provided by CiteAb. Source: Sci Rep, PMID: 31758055.