別品名 |
Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody
|
種由来 |
Rat
|
標識物 |
Unlabeled
|
適用 |
Western Blot Immuno Fluorescence
|
免疫動物 |
Chicken
|
抗体クラス |
IgG
|
交差種 |
Human Mouse Rat Bovine
|
GENE ID |
24547
|
Accession No.(Gene/Protein) |
NP_001020465, P02688
|
Gene Symbol |
MDV1
|
形状 |
液状
|
参考文献 |
Eylar EH, Brostoff S, Hashim G, Caccam J and Burnett P. (1971). Basic A1 protein of the myelin membrane: the complete amino acid sequence. J. Biol. Chem. 246:5770-5784. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. (2002). The myelin basic protein gene is expressed in differentiated blood cell lineages and in hematopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861.
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence Microscopy of Chicken Anti Myelin Basic Protein (MBP) Antibody. Tissue: Rat mixed neuron/glial cultures. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: MBP antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein chicken secondary antibody at 1:10,000 for 45 min at RT. Localization: MBP is cytoplasmic. Staining: chicken anti Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
Western Blot of Anti-Myelin Basic Protein (MBP) (Chicken) Antibody. Lane 1: rat cortex homogenate. Lane 2: none. Load: 10 ug per lane. Primary antibody: MBP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800TM chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 18kDa and 22kDa MBP protein. Other band(s): none.
|
|
Immunofluorescence Microscopy of Chicken Anti Myelin Basic Protein (MBP) Antibody. Tissue: Rat mixed neuron/glial cultures. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: MBP antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein chicken secondary antibody at 1:10,000 for 45 min at RT. Localization: MBP is cytoplasmic. Staining: chicken anti Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
|
|
|
メーカー |
品番 |
包装 |
RKL
|
200-901-D81
|
100 UL
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
-20℃
|
|