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Immunofluorescence Microscopy of Chicken Anti-Myelin Basic Protein (MBP) Antibody. Tissue: Rat mixed neuron/glial cultures. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: MBP antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Fluorescein chicken secondary antibody at 1:10,000 for 45 min at RT. Localization: MBP is cytoplasmic. Staining: chicken anti-Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
Western Blot of Anti-Myelin Basic Protein (MBP) (Chicken) Antibody. Lane 1: rat cortex homogenate. Lane 2: none. Load: 10 μg per lane. Primary antibody: MBP antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800？ chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~ 18kDa and 22kDa MBP protein. Other band(s): none.
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Immunofluorescence Microscopy of Chicken Anti-Myelin Basic Protein (MBP) Antibody. Tissue: Rat mixed neuron/glial cultures. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: MBP antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Fluorescein chicken secondary antibody at 1:10,000 for 45 min at RT. Localization: MBP is cytoplasmic. Staining: chicken anti-Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
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| 別品名 |
Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody
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| 交差種 |
Human
Mouse
Rat
Bovine
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| 適用 |
Western Blot
Immuno Fluorescence
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| 免疫動物 |
Chicken
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| 標識物 |
Unlabeled
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| GENE ID |
24547
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| Accession No.(Gene/Protein) |
NP_001020465.1, P02688
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| Gene Symbol |
Mbp
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| 参考文献 |
Eylar EH, Brostoff S, Hashim G, Caccam J and Burnett P. (1971). Basic A1 protein of the myelin membrane: the complete amino acid sequence. J. Biol. Chem. 246:5770-5784. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. (2002). The myelin basic protein gene is expressed in differentiated blood cell lineages and in hematopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861.
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| メーカー |
品番 |
包装 |
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RKL
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200-901-D81
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100 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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